LOCUS       sequence-140475-         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAL374 plasmid carrying the pck (NT) sgRNA, targeting the 
            non-template strand of pck, SpecR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Cleto S, Jensen JV, Wendisch VF, Lu TK
  TITLE     Corynebacterium glutamicum Metabolic Engineering with CRISPR 
            Interference (CRISPRi).
  JOURNAL   ACS Synth Biol. 2016 Feb 16.
  PUBMED    26829286
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Feb 16."
COMMENT     Sequence Label: pAL-pck_NT
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(41..46,47..62,63..69)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     misc_RNA        94..169
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ccgacatcat aacggttctg gcaaatattc tgaaatgagc tggacaatta atcatcggct
       61 cgtataatgt ccagttcagc agttccttat tctgttttag agctagaaat agcaagttaa
      121 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttatgga
      181 attggatccg tggaagacat ctgtcgaaga caccttgtgg tcgaagacac cttgtctctt
      241 cccagaggcg tggtcgcata ctaatttgta aacggccatg acgaaatagt cgtacggaac
      301 aaggagccac tagtctagaa ttcggtgagg ttatggcgga gggttgcgag gtctaggaga
      361 acagaggaag tcatgctttg aagcatataa gctgccctgc ccctcaaggt tttcttcaag
      421 tgaggtttta tctaactgcc taacggcagg ggaaccgtat attgcttacg gtatgagacc
      481 ccttaaacgt ccggatagtc accgctcttc tttagctccg cgacatgcct agcaaccgtg
      541 gcgcgagaga ctcctacctc tgcccctatt tcagcccacg tgggaactgt ccctgtctgg
      601 aaatactgat cgttcaccat ttggctaata cgggacttcg tagatcgtcc ttgagccttt
      661 ttcttacggt gcgtcttttc aagcttcgac ctttgtgctt gcgcatattt gccctcgggg
      721 tctgttttcc agcgttgtgc ggctttttgt ccgcctctgc gtcccatcgt ggccaaggct
      781 ttccgctcgc tgctggtggc tttacctggt gcgttagagc cgctgtaggt ctcgctcttg
      841 gattgggcga catacccgcg cacgcgcctt gccatggttt ggcggtcgcg catgggtggc
      901 atctcgttgt cgcggcctgc accgccgtg
//
