LOCUS       sequence-140476-         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAL374 plasmid carrying the pck (NT) sgRNA, targeting the 
            non-template strand of pck, SpecR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Cleto S, Jensen JV, Wendisch VF, Lu TK
  TITLE     Corynebacterium glutamicum Metabolic Engineering with CRISPR 
            Interference (CRISPRi).
  JOURNAL   ACS Synth Biol. 2016 Feb 16.
  PUBMED    26829286
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Feb 16."
COMMENT     Sequence Label: pAL-pck_NT
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(41..47,48..63,64..69))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 tttctagctc taaaacagaa taaggaactg ctgaactgga cattatacga gccgatgatt
       61 aattgtccag ctcatttcag aatatttgcc agaaccgtta tgatgtcggc gcaaaaaaca
      121 ttatccagaa cgggagtgcg ccttgagcga cacgaattat gcagtgattt acgacctgca
      181 cagccatacc acagcttccg atggctgcct gacgccagaa gcattggtgc accgtgcagt
      241 cgatgataag ctgtcaaacc agatcaattc gcgctaactt acattaattg cgttgcgctc
      301 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg aaacgcggtt acaattcgcc
      361 cttccaattc gcccttgcaa cgcgtttcga attatttgcc gactaccttg gtgatctcgc
      421 ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt
      481 cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg gccggcaggc
      541 gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt actgcgctgt
      601 accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag tcgggcggcg
      661 agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca ggaaccggat
      721 caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt gcttttgtca
      781 gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat
      841 tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac ggaatgatgt
      901 cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct ccag
//
