LOCUS       sequence-140477-         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAL374 plasmid carrying the pyk (T) sgRNA targeting the template 
            strand of pyk, SpecR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Cleto S, Jensen JV, Wendisch VF, Lu TK
  TITLE     Corynebacterium glutamicum Metabolic Engineering with CRISPR 
            Interference (CRISPRi).
  JOURNAL   ACS Synth Biol. 2016 Feb 16.
  PUBMED    26829286
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Feb 16."
COMMENT     Sequence Label: pAL-pyk_T
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aacctgggcc tagggtacat acaatctcat tatacgagcc gatgttaatt gtcaaagctc
       61 atttcgaata tttgccagaa ccgttatgat gtcggcgcaa aaaacattat ccagaacggg
      121 agtgcgcctt gagcgacacg aattatgcag tgatttacga cctgcacagc cataccacag
      181 cttccgatgg ctgcctgacg ccagaagcat tggtgcaccg tgcagtcgat gataagctgt
      241 caaaccagat caattcgcgc taacttacat taattgcgtt gcgctcactg cccgctttcc
      301 agtcgggaaa cctgtcgtgc cagctgaaac gcggttacaa ttcgcccttc caattcgccc
      361 ttgcaacgcg tttcgaatta tttgccgact accttggtga tctcgccttt cacgtagtgg
      421 acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg tccaagataa
      481 gcctgtctag cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag
      541 tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac
      601 aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt
      661 aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc
      721 gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga
      781 tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg ctgccattct
      841 ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcacaaca
      901 atg
//
