LOCUS       sequence-140478-        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAL374 plasmid carrying the pgi (T) sgRNA, targeting the template 
            strand of pgi, SpecR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Cleto S, Jensen JV, Wendisch VF, Lu TK
  TITLE     Corynebacterium glutamicum Metabolic Engineering with CRISPR 
            Interference (CRISPRi).
  JOURNAL   ACS Synth Biol. 2016 Feb 16.
  PUBMED    26829286
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Feb 16."
COMMENT     Sequence Label: pAL-pgi_T
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(60..65,66..80,81..87)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     misc_RNA        112..187
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ggaatggttt tttgcgccga catcataacg ggttctggca aatattctga aatgagctgt
       61 tgacaattaa tcacggctcg tataatgtga ccgatcatta ctcaaacttc cgttttagag
      121 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
      181 tcggtgcttt tttatggaat tggatccgtg gaagacatct gtcgaagaca ccttgtggtc
      241 gaagacacct tgtctcttcc cagaggcgtg gtcgcatact aatttgtaaa cggccatgac
      301 gaaatagtcg tacggaacaa ggagccacta gtctagaatt cggtgaggtt atggcggagg
      361 gttgcgaggt ctaggagaac agaggaagtc atgctttgaa gcatataagc tgccctgccc
      421 ctcaaggttt tcttcaagtg aggttttatc taactgccta acggcagggg aaccgtatat
      481 tgcttacggt atgagacccc ttaaacgtcc ggatagtcac cgctcttctt tagctccgcg
      541 acatgcctag caaccgtggc gcgagagact cctacctctg cccctatttc agcccacgtg
      601 ggaactgtcc ctgtctggaa atactgatcg ttcaccattt ggctaatacg ggacttcgta
      661 gatcgtcctt gagccttttt cttacggtgc gtcttttcaa gcttcgacct ttgtgcttgc
      721 gcatatttgc cctcggggtc tgttttccag cgttgtgcgg ctttttgtcc gcctctgcgt
      781 cccatcgtgg ccaaggcttt ccgctcgctg ctggtggctt tacctggtgc gttagagccg
      841 ctgtaggtct cgctcttgga ttgggcgaca tacccgcgca cgcgccttgc catggtttgg
      901 cggtcgcgca tgggtggcat ctcgttgtcg cggcctgcac cgccgtgggt gtgtgcgacg
      961 ttgtangcgt gctcatangc gtcnatgatt gctgcntctg tcagncgttg gcctngctgg
     1021 cnc
//
