LOCUS       sequence-140479-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAL374 plasmid carrying the pgi (T) sgRNA, targeting the template 
            strand of pgi, SpecR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Cleto S, Jensen JV, Wendisch VF, Lu TK
  TITLE     Corynebacterium glutamicum Metabolic Engineering with CRISPR 
            Interference (CRISPRi).
  JOURNAL   ACS Synth Biol. 2016 Feb 16.
  PUBMED    26829286
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Feb 16."
COMMENT     Sequence Label: pAL-pgi_T
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(36..42,43..57,58..63))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 agctctaaaa cggaagtttg agtaatgatc ggtcacatta tacgagccgt gattaattgt
       61 caacagctca tttcagaata tttgccagaa ccgttatgat gtcggcgcaa aaaacattat
      121 ccagaacggg agtgcgcctt gagcgacacg aattatgcag tgatttacga cctgcacagc
      181 cataccacag cttccgatgg ctgcctgacg ccagaagcat tggtgcaccg tgcagtcgat
      241 gataagctgt caaaccagat caattcgcgc taacttacat taattgcgtt gcgctcactg
      301 cccgctttcc agtcgggaaa cctgtcgtgc cagctgaaac gcggttacaa ttcgcccttc
      361 caattcgccc ttgcaacgcg tttcgaatta tttgccgact accttggtga tctcgccttt
      421 cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg
      481 tccaagataa gcctgtctag cttcaagtat gacgggctga tactgggccg gcaggcgctc
      541 cattgcccag tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca
      601 aatgcgggac aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt
      661 ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa
      721 gagttcctcc gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa
      781 gatagccaga tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg
      841 ctgccattct ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc
      901 gtgcacaaca atggtgactt ctacagcgcg
//
