LOCUS       sequence-140482-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAL374 plasmid carrying the pgi (NT) sgRNA, targeting the 
            non-template strand of pgi, SpecR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Cleto S, Jensen JV, Wendisch VF, Lu TK
  TITLE     Corynebacterium glutamicum Metabolic Engineering with CRISPR 
            Interference (CRISPRi).
  JOURNAL   ACS Synth Biol. 2016 Feb 16.
  PUBMED    26829286
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Feb 16."
COMMENT     Sequence Label: pAL-pgi_NT
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(27..33,34..49,50..54))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 acatcattac tcaaacttcc aggcaacatt atacgagccg atgattaatt gtcagcagct
       61 catttcagaa tatttgccag aaccgttatg atgtcggcgc aaaaaacatt atccagaacg
      121 ggagtgcgcc ttgagcgaca cgaattatgc agtgatttac gacctgcaca gccataccac
      181 agcttccgat ggctgcctga cgccagaagc attggtgcac cgtgcagtcg atgataagct
      241 gtcaaaccag atcaattcgc gctaacttac attaattgcg ttgcgctcac tgcccgcttt
      301 ccagtcggga aacctgtcgt gccagctgaa acgcggttac aattcgccct tccaattcgc
      361 ccttgcaacg cgtttcgaat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
      421 ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
      481 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
      541 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
      601 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
      661 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
      721 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
      781 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
      841 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
      901 caatggtgac ttctacagcg cgga
//
