LOCUS       sequence-140487-         690 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses in E. coli, Bacillus cereus  NP_831800 CDP-CHase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Duong-Ly KC, Gabelli SB, Xu W, Dunn CA, Schoeffield AJ, Bessman MJ, 
            Amzel LM
  TITLE     The Nudix hydrolase CDP-chase, a CDP-choline pyrophosphatase, is an 
            asymmetric dimer with two distinct enzymatic activities.
  JOURNAL   J Bacteriol. 2011 Jul;193(13):3175-85. doi: 10.1128/JB.00089-11. 
            Epub 2011 Apr 29.
  PUBMED    21531795
REFERENCE   2  (bases 1 to 690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JB.00089-11"; journalName: "J Bacteriol"; date: "2011-07"; 
            volume: "193"; issue: "13"; pages: "3175-85"
COMMENT     Sequence Label: pET24a_CDP-CHase
FEATURES             Location/Qualifiers
     source          1..690
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..39)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg ccccaagctt
       61 gtcgacggag ctcgaattcg gatccttaat ctattaattt ctctttctgc gggtctttca
      121 tgtaggcaaa catctcttta atttggtctt ctgtatttct agcaattgat aaattaggca
      181 attcattttc accaaaaaac tccacctctt ctgtttcaat actagttttc ttttctccac
      241 caataatctc gcatccaatg aaaatcttat atacatgcgt cgccgatgga gatggttgat
      301 gtttttcttt atcaaatata gctagtaact taaaatgatc tacctcataa ccagtctctt
      361 cgaaaacttc ttttgctgca acttcggttg gcgtgtaacc gacatcagcc catccacctg
      421 gcaatgccca tttcccatcg cttttttctt tcacaaataa taacttttca ttttgaaaaa
      481 caaccgctct tatatcaact ttaggtgttt gatagcccgt ctcacttgca aataatttct
      541 ctacgacttc ccaatctgtt ttcgtgtaat gtgacatcat tgaaatagaa atatctcgta
      601 attgctggaa acgctcaata tcatacacat ccttagaata cgttaaaccc gcttgagcta
      661 tagattgtat ttgttttacc caatcaatcc
//
