LOCUS       sequence-140492-        9926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human codon optimized plasmid to express arC9 t2a mCherry w/o NLS 
            and a sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9926)
  AUTHORS   Oakes BL, Nadler DC, Flamholz A, Fellmann C, Staahl BT, Doudna JA, 
            Savage DF
  TITLE     Profiling of engineering hotspots identifies an allosteric 
            CRISPR-Cas9 switch.
  JOURNAL   Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528.
  PUBMED    27136077
REFERENCE   2  (bases 1 to 9926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528."
COMMENT     Sequence Label: pBLO1811_arC9_noNLS_human
FEATURES             Location/Qualifiers
     source          1..9926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        651..899
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     651..671
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     822..841
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        1100..1385
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        1387..1664
                     /label=chicken beta-actin promoter
     intron          1665..1893
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     CDS             1908..1931
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4308..6806
                     /codon_start=1
                     /product="C-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(C)
                     /translation="KPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEI
                     SGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTY
                     AHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIH
                     DDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPE
                     NIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYL
                     QNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEV
                     VKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQI
                     LDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVV
                     GTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITL
                     ANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIL
                     PKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIME
                     RSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALP
                     SKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLD
                     KVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATL
                     IHQSITGLYETRIDLSQLGGD"
     CDS             6828..6881
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6882..7592
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7027..7045)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(7617..7634)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7623..7830
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   7839..7979
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   7839..7968
                     /label=AAV2 ITR
     rep_origin      8054..8509
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8141..8160)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8351..8372
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8526..8545)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8645..8667
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8705..8723)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8791..8895
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8896..8964,8965..9756)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9114..9133)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt gagggcctat
      661 ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa
      721 ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat
      781 ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg
      841 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg
      901 ggtcttcgag aagacctgtt taagagctat gctggaaaca gcatagcaag tttaaataag
      961 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttg ttttagagct
     1021 agaaatagca agttaaaata aggctagtcc gtttttagcg cgtgcgccaa ttctgcagac
     1081 aaatggctct agaggtaccc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     1141 ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc cattgacgtc
     1201 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     1261 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tgtgcccagt
     1321 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     1381 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
     1441 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
     1501 ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga
     1561 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
     1621 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgacgc
     1681 tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg
     1741 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag
     1801 ctgagcaaga ggtaagggtt taagggatgg ttggttggtg gggtattaat gtttaattac
     1861 ctggagcacc tgcctgaaat cacttttttt caggttggac cggtatggat tacaaagacg
     1921 atgacgataa ggacaagaag tacagcatcg gcctggacat cggcaccaac tctgtgggct
     1981 gggccgtgat caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca
     2041 ccgaccggca cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa
     2101 cagccgaggc cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc
     2161 ggatctgcta tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct
     2221 tccacagact ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca
     2281 tcttcggcaa catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc
     2341 tgagaaagaa actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc
     2401 tggcccacat gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca
     2461 acagcgacgt ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg
     2521 aaaaccccat caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca
     2581 agagcagacg gctggaaaat ctgatcgccc agctgcccgg cgcatctcca gcaaacagtc
     2641 ttgccttgag tctcacagca gaccagatgg tgtcagcact gctcgatgcg gagccaccta
     2701 tactctactc tgaatacgac ccaaccagac cgttcagcga ggcttcaatg atgggactcc
     2761 tgactaatct ggcggacagg gaacttgtgc acatgatcaa ttgggccaaa cgggttcccg
     2821 gttttgttga ccttaccctg catgatcagg ttcatctgtt ggaatgtgca tggctcgaaa
     2881 tacttatgat tgggctcgta tggaggtcca tggagcaccc tggcaagctc ctgtttgctc
     2941 caaatctctt gttggatcgg aatcagggta aatgtgtcga aggaatggta gagatttttg
     3001 atatgctcct ggcaacctcc agtcggttcc gaatgatgaa tctgcagggg gaggagttcg
     3061 tgtgtctgaa atctattatc ctgcttaatt ccggcgttta tacttttctg tcatctacac
     3121 tgaagtccct ggaggagaag gaccacattc atcgcgtgtt ggacaagata accgacacac
     3181 tcatccatct gatggctaag gcagggctga ccctgcaaca gcaacaccag aggcttgccc
     3241 aactgttgct cattctgagc cacatcaggc atatgtccaa taaaggaatg gaacacctgt
     3301 acagcatgaa gtgtaagaac gtagtgccac tctatgatct tcttttggag atgctggacg
     3361 ctcacaggct ccacgcacca cctgcatctc caggcgagaa gaagaatggc ctgttcggca
     3421 acctgattgc cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg
     3481 aggatgccaa actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg
     3541 cccagatcgg cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca
     3601 tcctgctgag cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct
     3661 ctatgatcaa gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc
     3721 ggcagcagct gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg
     3781 ccggctacat tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc
     3841 tggaaaagat ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc
     3901 ggaagcagcg gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc
     3961 acgccattct gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga
     4021 tcgagaagat cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca
     4081 gcagattcgc ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg
     4141 aagtggtgga caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata
     4201 agaacctgcc caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg
     4261 tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc
     4321 tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga
     4381 ccgtgaagca gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa
     4441 tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa
     4501 ttatcaagga caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg
     4561 tgctgaccct gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg
     4621 cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg
     4681 gcaggctgag ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc
     4741 tggatttcct gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg
     4801 acagcctgac ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc
     4861 tgcacgagca cattgccaat ctggccggca gccccgccat taagaagggc atcctgcaga
     4921 cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg
     4981 tgatcgaaat ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga
     5041 gaatgaagcg gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc
     5101 ccgtggaaaa cacccagctg cagaacgaga agctgtacct gtactacctg cagaatgggc
     5161 gggatatgta cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacc
     5221 atatcgtgcc tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa
     5281 gcgacaagaa ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga
     5341 agaactactg gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc
     5401 tgaccaaggc cgagagaggc ggcctgagcg aactggataa ggccggcttc atcaagagac
     5461 agctggtgga aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga
     5521 acactaagta cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt
     5581 ccaagctggt gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca
     5641 actaccacca cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa
     5701 agtaccctaa gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga
     5761 agatgatcgc caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca
     5821 gcaacatcat gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc
     5881 ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt
     5941 ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg
     6001 tgcagacagg cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gataagctga
     6061 tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgacagc cccaccgtgg
     6121 cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg
     6181 tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg
     6241 actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta
     6301 agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct gccggcgaac
     6361 tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca
     6421 gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg
     6481 aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag
     6541 tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata
     6601 agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag
     6661 cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac accagcacca
     6721 aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga
     6781 tcgacctgtc tcagctggga ggcgacggat ccgaatccgg cagtggagag ggcagaggaa
     6841 gtctgctaac atgcggtgac gtcgaggaga atcctggccc aatggtgagc aagggcgagg
     6901 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
     6961 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     7021 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
     7081 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
     7141 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     7201 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
     7261 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aaaaccatgg
     7321 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
     7381 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
     7441 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca
     7501 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
     7561 ccaccggcgg catggacgag ctgtacaagt aagaattcct agagctcgct gatcagcctc
     7621 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     7681 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     7741 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     7801 ttgggaagag aatagcaggc atgctgggga gcggccgcag gaacccctag tgatggagtt
     7861 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
     7921 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
     7981 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
     8041 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     8101 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     8161 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     8221 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
     8281 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     8341 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat
     8401 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     8461 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
     8521 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     8581 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     8641 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     8701 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     8761 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     8821 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     8881 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     8941 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     9001 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     9061 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     9121 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     9181 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     9241 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     9301 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     9361 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     9421 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     9481 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     9541 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     9601 ggtgagcgtg gaagccgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     9661 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     9721 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     9781 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     9841 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     9901 cccgtagaaa agatcaaagg atcttc
//
