LOCUS       sequence-140498-         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TL9 RBS (AAAAAG).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-TL9
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..21
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(45..102)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(130..157)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(173..244)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(300..320)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgcagcgagt cagtgagcga ggaagcctgc ataacgcgaa gtaatctttt cggttttaaa
       61 gaaaaagggc agggtggtga caccttgccc ttttttgccg gactgcagcg tctctggcaa
      121 acatataaac gcagaaaggc ccacccgaag gtgagccagt gtgactctag tagagagcgt
      181 tcaccgacaa acaacagata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta
      241 tttgatgcct ggtcgattat ttgtatagtt catccatgcc atgtgtaatc ccagcagctg
      301 ttacaaactc aagaaggacc atgtgatctc tcttttcgtt gggatctttc gaaagggcag
      361 attgtgtgga caggtaatgg ttgtctggta aaaggacagg gccatcgcca attggagtat
      421 tttgttgata atggtctgct agttgaacgc ttccatcttc aatgttgtgt ctaattttga
      481 agttaacttt gattccattc ttttgtttgt ctgccatgat gtatacattg tgtgagttat
      541 agttgtattc caatttgtgt ccaagaatgt ttccatcttc tttaaaatca atacctttta
      601 actcgattct attaacaagg gtatcacctt caaacttgac ttcagcacgt gtcttgtagt
      661 tcccgtcatc tttgaaaaat atagttcttt cctgtacata accttcgggc atggcactct
      721 tgaaaaagtc atgctgtttc atatgatctg ggtatctcgc aaagcattga acaccataac
      781 cgaaagtagt gacaagtgtt ggccatggaa caggtagttt tccagtagtg caaataaatt
      841 taagggtaag ttttccgtat gttgcatcac cttcaccctc tccactgaca gaa
//
