LOCUS       sequence-140500-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BBa_J23100 promoter (iGEM collection).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 113 to 147)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-BBa_J23100
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(22..79)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     promoter        complement(113..147)
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     misc_feature    175..194
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      197..240
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(244..262)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(490..509)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 agcctgcata acgcgaagta atcttttcgg ttttaaagaa aaagggcagg gtggtgacac
       61 cttgcccttt tttgccggac tgcagctaat gagacggcat gggtctctgt acgctagcac
      121 tgtacctagg actgagctag ccgtcaaata gtgagaccta tgcgtctcta gattctagaa
      181 gcggccgcga attccagaaa tcatccttag cgaaagctaa ggattttttt tatctgaaat
      241 tctgcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
      301 cgtcaggtgg cactcgagtt gatcgggcac gtaagaggtt ccaactttca ccataatgaa
      361 ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct
      421 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
      481 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
      541 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
      601 attcacattc ttgcccgcct gatgaatgct catccggaat ttcgtatggc aatgaaagac
      661 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
      721 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
      781 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
      841 gagaatatgt ttttcgtg
//
