LOCUS       sequence-140501-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  T7 RNA polymerase PCR cloned from BL21 (DE3) genomic DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-T7RNAP
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..21
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(45..102)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
ORIGIN
        1 cgcagcgagt cagtgagcga ggaagcctgc ataacgcgaa gtaatctttt cggttttaaa
       61 gaaaaagggc agggtggtga caccttgccc ttttttgccg gactgcagct aatgagacgg
      121 catgggtctc ttcgattacg cgaacgcgaa gtccgactct aagatgtcac ggaggttcaa
      181 gttaccttta gccggaagtg ctggcatttt gtccaattga gactcgtgca actggtcagc
      241 gaactggtcg tagaaatcag ccagtacatc acaagactca tatgtgtcaa ccatagtttc
      301 gcgcactgct ttgaacaggt tcgcagcgtc agccggaatg gtaccgaagg agtcgtgaat
      361 cagtgcaaaa gattcgattc cgtacttctc gtgtgcccac actacagtct tacgaaggtg
      421 gctaccgtct tggctgtgta caaagttagg agcgatacca gactcctgtt tgtgtgcatc
      481 aatctcgcta tctttgttgg tgttaatggt aggctgtaag cggaactgac cgaggaacat
      541 caggttcaag cgcgtctgaa taggcttctt gtattcctgc cacacaggga aaccatcagg
      601 agttacccaa tgcacagcgc aacgcttgcg aagaatctct ccagtcttct tatctttgac
      661 ctcagcagcc agcagcttag cagcagactt aagccagttc attgcttcaa ccgcagctac
      721 caccgtcacg ctcacagatt cccaaatcag cttagccatg tatccagcag cctgattcgg
      781 ctgagtgaac atcagaccct tgccggaatc aatagctggc tgaatgg
//
