LOCUS       sequence-140502-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 1 Destination vector - Position A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pTU1-A-lacZ
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             382..393
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(402..418)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(402..418)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(415..437)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    426..442
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    479..498
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      501..544
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(548..566)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        634..738
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcagtgagc gaggaagcct gcagcgtctc tggcaaacat gagaccgcat gcggatccac
       61 tagtgcgaaa aaaccccgcc ctgtcagggg cggggttttt tgcgttagtt gttagacagc
      121 ggcggcagca gccaagacag tttacgttcc ggtttggtca gcagacggat cagagaacgc
      181 agctgctggg acggacggtc ggtacgagct tcttcagagt tacgccaaga agcgaacggc
      241 gggtgagcag ccagacggtt cagctgggta acacccgggt tttcccagtc acgacgttgc
      301 agaacaacag ccagagagtt acccggagaa gaagagatag cgtgcggaac ttccagcagc
      361 agtttcgggt cacccatgtc ctttctcctc tttgtaccat ggtcatagct gtttcctgtg
      421 tgaaattgtt atccgctcac aacatatgtc tagaggtctc tatagagatt gagacgttct
      481 agaagcggcc gcgaattcca gaaatcatcc ttagcgaaag ctaaggattt tttttatctg
      541 aaattctgcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
      601 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
      661 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      721 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tcccttt
//
