LOCUS       sequence-140503-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  L-lactate repressor (LldR) from E. coli MG1655.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-lldR
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..21
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(45..102)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
ORIGIN
        1 cgcagcgagt cagtgagcga ggaagcctgc ataacgcgaa gtaatctttt cggttttaaa
       61 gaaaaagggc agggtggtga caccttgccc ttttttgccg gactgcagct aatgagacgg
      121 catgggtctc ttcgaggatc cttatgcgtt tttctccctc gaatgctcat tatgctcacc
      181 gggcaggcgg gtaatccgtg cgtggcgagc ctgatcttca tcgaatcgtt tcatggtggt
      241 gtgaacaaaa ctaaggtgcg ccatcattgc tttacgcgcc ccgtcagcat caccggcaaa
      301 aatggcgtca atgacagcct gatgttgttc ggtcagttgt gaaaaaaccg gtggcaccag
      361 atacatccgc tgacggctat gcttcactga ggattgcagg acatcgaaga aaccgcgcat
      421 ggtttgcagc agcacgatgt tatgtgaggc ttcggcaatc gccagatgaa aacgaacgtc
      481 cgcttgtgag gcgatatccg ggtcttcact tagcgttgct tcaaagcaaa gctgaatctt
      541 ttctttgtcg ccaggtgtgg cgcgcattgc cgcatgccat gcggtgctgg cttcaatggc
      601 gtagcgggct tccagaatat cgaaactgta atccggatca tcggccatca gtgtttttag
      661 cggctggacg atgttttgct ccgaccatgt gtcatgacgc cagcgaataa acgtcccgcc
      721 gccgcgtcga ctgagcagca cgccttcact caccagtttt gccagcgcct cgcgcagtga
      781 attacgtgat acgccgagtt gcatcgccag ttggcgctca gcgggcaact tcatgcccgc
      841 tt
//
