LOCUS       sequence-140505-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  L-Lactate inducible promoter from E. coli MG1655.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-Bba_K822000
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..24
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(48..105)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    501..520
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      523..566
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(570..588)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(816..835)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gagcgcagcg agtcagtgag cgaggaagcc tgcataacgc gaagtaatct tttcggtttt
       61 aaagaaaaag ggcagggtgg tgacaccttg cccttttttg ccggactgca gctaatgaga
      121 cggcatgcgg tctctgtacg gagtccacgc acatcgtgta atgagtctgt tgctcatctc
      181 cttgtcacac gttgtgtaaa agtggcactg ccaattgtgt tgtgtaggga taatgacaat
      241 gaatggttaa ctaaatgtta tgtattggca acattgatgt gagttttaat tgggaaattg
      301 tgaaccagag gtcagataag cgaagaattg gtagggccaa ttcttgtggg gggagatgtc
      361 tggtaaagaa aagggcaatc ctcatcagag aattgccctt aggttgttga tccagagcgc
      421 cttatcttgc cggatgcggc gtgaacacct tactcggcct ataggaatgt gatagtgaga
      481 cccatatgcg tctctagatt ctagaagcgg ccgcgaattc cagaaatcat ccttagcgaa
      541 agctaaggat tttttttatc tgaaattctg cctcgtgata cgcctatttt tataggttaa
      601 tgtcatgata ataatggttt cttagacgtc aggtggcact cgagttgatc gggcacgtaa
      661 gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat
      721 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
      781 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
      841 gtacctataa ccagaccgtt cagctggata ttacggc
//
