LOCUS       sequence-140506-         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTU2-A backbone, with RFP negative selection and colE1 (medium-copy)
            origin of replication.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pTU2-A-RFP (colE1 origin)
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..21
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(45..102)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(143..170)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(186..257)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgcagcgagt cagtgagcga ggaagcctgc ataacgcgaa gtaatctttt cggttttaaa
       61 gaaaaagggc agggtggtga caccttgccc ttttttgccg gactgcaggg tctctgtacc
      121 taatgagacg gcatgctata aacgcagaaa ggcccacccg aaggtgagcc agtgtgactc
      181 tagtagagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga
      241 gcctttcgtt ttatttgatg cctggctcta gtagcgatct acactagcac tatcagcgtt
      301 attaagcacc ggtggagtga cgaccttcag cacgttcgta ctgttcaacg atggtgtagt
      361 cttcgttgtg ggaggtgatg tccagtttga tgtcggtttt gtaagcaccc ggcagctgaa
      421 ccggtttttt agccatgtag gtggttttaa cttcagcgtc gtagtgacca ccgtctttca
      481 gtttcagacg cattttgatt tcacctttca gagcaccgtc ttccgggtac atacgttcgg
      541 tggaagcttc ccaacccatg gtttttttct gcataaccgg accgtcggac gggaagttgg
      601 taccacgcag tttaactttg tagatgaact caccgtcttg cagggaggag tcctgggtaa
      661 cggtaacaac accaccgtct tcgaagttca taacacgttc ccatttgaaa ccttccggga
      721 aggacagttt caggtagtcc gggatgtcag ccgggtgttt aacgtaagct ttggaaccgt
      781 actggaactg cggg
//
