LOCUS       sequence-140507-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Secondary module (BpiI) site in pTU2-a backbone with colE1 
            (medium-copy) origin of replication.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pTU2S-b (colE1 origin)
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..21
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(45..102)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    155..176
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     primer_bind     complement(407..426)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cgcagcgagt cagtgagcga ggaagcctgc ataacgcgaa gtaatctttt cggttttaaa
       61 gaaaaagggc agggtggtga caccttgccc ttttttgccg gactgcaggg tctctgtacc
      121 ttctgagacg gtgcgtctct agatatagtg agacctctag aagcggccgc gaattcgtac
      181 ttgtcttcgc atgcgtgcat atggaagact tataggacgt caggtggcac tcgagttgat
      241 cgggcacgta agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt
      301 ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
      361 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
      421 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
      481 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
      541 gaatgctcat ccggaatttc gtatggcaat gaaagacggt gagctggtga tatgggatag
      601 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
      661 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
      721 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtgtcagc
      781 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
      841 cgcccc
//
