LOCUS       sequence-140511-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTU2-A backbone, with RFP negative selection and p15A (low-copy) 
            origin of replication.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pTU2-A-RFP (p15A origin)
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..25
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(49..106)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(147..174)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(190..261)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgagcgcagc gagtcagtga gcgaggaagc ctgcataacg cgaagtaatc ttttcggttt
       61 taaagaaaaa gggcagggtg gtgacacctt gccctttttt gccggactgc agggtctctg
      121 tacctaatga gacggcatgc tataaacgca gaaaggccca cccgaaggtg agccagtgtg
      181 actctagtag agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga
      241 ctgagccttt cgttttattt gatgcctggc tctagtagcg atctacacta gcactatcag
      301 cgttattaag caccggtgga gtgacgacct tcagcacgtt cgtactgttc aacgatggtg
      361 tagtcttcgt tgtgggaggt gatgtccagt ttgatgtcgg ttttgtaagc acccggcagc
      421 tgaaccggtt ttttagccat gtaggtggtt ttaacttcag cgtcgtagtg accaccgtct
      481 ttcagtttca gacgcatttt gatttcacct ttcagagcac cgtcttccgg gtacatacgt
      541 tcggtggaag cttcccaacc catggttttt ttctgcataa ccggaccgtc ggacgggaag
      601 ttggtaccac gcagtttaac tttgtagatg aactcaccgt cttgcaggga ggagtcctgg
      661 gtaacggtaa caacaccacc gtcttcgaag ttcataacac gttcccattt gaaaccttcc
      721 gggaaggaca gtttcaggta gtccgggatg tcagccgggt gtttaacgta agctttggaa
      781 ccgtactgga actgcgg
//
