LOCUS       sequence-140517-         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 1 Destination vector - Position D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pTU1-D-RFP
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(57..84)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(100..171)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gcgagtcagt gagcgaggaa gcctgcagcg tctctctaaa acatgagacc tataaacgca
       61 gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca ccgacaaaca
      121 acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcctggc
      181 tctagtagcg atctacacta gcactatcag cgttattaag caccggtgga gtgacgacct
      241 tcagcacgtt cgtactgttc aacgatggtg tagtcttcgt tgtgggaggt gatgtccagt
      301 ttgatgtcgg ttttgtaagc acccggcagc tgaaccggtt ttttagccat gtaggtggtt
      361 ttaacttcag cgtcgtagtg accaccgtct ttcagtttca gacgcatttt gatttcacct
      421 ttcagagcac cgtcttccgg gtacatacgt tcggtggaag cttcccaacc catggttttt
      481 ttctgcataa ccggaccgtc ggacgggaag ttggtaccac gcagtttaac tttgtagatg
      541 aactcaccgt cttgcaggga ggagtcctgg gtaacggtaa caacaccacc gtcttcgaag
      601 ttcataacac gttcccattt gaaaccttcc gggaaggaca gtttcaggta gtccgggatg
      661 tcagccgggt gtttaacgta agctttggaa ccgtactgga actgcgggga caggatgtcc
      721 caagcgaacg gcagcggacc acctttggta actttcagtt tagcggtctg ggtaccttcg
      781 tacggacgac cttcaccttc accttcgatt tcga
//
