LOCUS       sequence-140530-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 1 Destination vector - Position D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pTU1-D-RFP
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(22..65)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    68..87
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     protein_bind    212..233
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(248..253,254..271,272..278)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    286..302
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             320..331
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg
       61 atttctggaa ttcgcggccg cttctagaac gtctcagaag ctatagagac ccaatacgca
      121 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
      181 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
      241 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
      301 aatttcacac atactagaga aagaggagaa atactagatg gcttcctccg aagacgttat
      361 caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga
      421 aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt
      481 taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg
      541 ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc
      601 ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac
      661 ccaggactcc tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa
      721 cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag cttccaccga
      781 acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa
      841 agacggtggt cactacgacg ctgaagttaa a
//
