LOCUS       sequence-140536-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Backbone vector with internal BsmBI removed by site-directed 
            mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(22..65)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    68..87
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      132..189
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(213..230)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(464..483)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg
       61 atttctggaa ttcgcggccg cttctagaat ctagagacgc atatgcacgc atgccgtctc
      121 attagctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa
      181 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc
      241 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      301 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      361 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa
      421 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      481 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      541 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      601 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      661 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
      721 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
      781 acagagttct tgaagtggtg gcctaactac ggctacacta g
//
