LOCUS       sequence-140538-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry vector for ORF's - PCR, gene synthesis or sub-clone using 
            NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. 
            Stop codon must be included upstream of chosen 3' restriction site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-ORF
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..19
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(23..66)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    69..88
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      172..229
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(253..270)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(504..523)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
       61 gatttctgga attcgcggcc gcttctagaa tctagagacg cataggtctc acatatgggt
      121 tccatggggc acggatcctc gaagagaccc atgccgtctc attagctgca gtccggcaaa
      181 aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt
      241 tatgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
      301 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      361 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      421 gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      481 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      541 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      601 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
      661 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
      721 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
      781 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
      841 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
//
