LOCUS       sequence-140548-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 2 Destination vector - Position A - Assembly of 4-5 TU's from 
            Level 1-ABCD or ABCDE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pTU2-A-RFP
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..19
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(23..66)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    69..90
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    224..245
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(260..265,266..283,284..290)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    298..314
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             332..343
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
       61 gatttctgga attcgcggcc gcttctagag gtctcactat atctagagac gtctagacat
      121 atgcaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
      181 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
      241 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
      301 tgagcggata acaatttcac acatactaga gaaagaggag aaatactaga tggcttcctc
      361 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
      421 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
      481 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
      541 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
      601 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt
      661 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
      721 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
      781 agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg
      841 tctgaaactg aaagacggtg gtcactacga cgctgaagtt aa
//
