LOCUS       sequence-140549-         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAL374 plasmid carrying the pck (T) sgRNA targeting, the template 
            strand of pck, SpecR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Cleto S, Jensen JV, Wendisch VF, Lu TK
  TITLE     Corynebacterium glutamicum Metabolic Engineering with CRISPR 
            Interference (CRISPRi).
  JOURNAL   ACS Synth Biol. 2016 Feb 16.
  PUBMED    26829286
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Feb 16."
COMMENT     Sequence Label: pAL-pck_T
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(20..25,26..41,42..48)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     misc_RNA        73..148
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 aatattctga aatgagctgt tgacaattaa tcatcggctc gtataatgag ggcgaggcgc
       61 cgaccaagaa tagttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
      121 acttgaaaaa gtggcaccga gtcggtgctt ttttatggaa ttggatccgt ggaagacatc
      181 tgtcgaagac accttgtggt cgaagacacc ttgtctcttc ccagaggcgt ggtcgcatac
      241 taatttgtaa acggccatga cgaaatagtc gtacggaaca aggagccact agtctagaat
      301 tcggtgaggt tatggcggag ggttgcgagg tctaggagaa cagaggaagt catgctttga
      361 agcatataag ctgccctgcc cctcaaggtt ttcttcaagt gaggttttat ctaactgcct
      421 aacggcaggg gaaccgtata ttgcttacgg tatgagaccc cttaaacgtc cggatagtca
      481 ccgctcttct ttagctccgc gacatgccta gcaaccgtgg cgcgagagac tcctacctct
      541 gcccctattt cagcccacgt gggaactgtc cctgtctgga aatactgatc gttcaccatt
      601 tggctaatac gggacttcgt agatcgtcct tgagcctttt tcttacggtg cgtcttttca
      661 agcttcgacc tttgtgcttg cgcatatttg ccctcggggt ctgttttcca gcgttgtgcg
      721 gcttt
//
