LOCUS       sequence-140551-         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAL374 plasmid carrying the rfp (T) sgRNA targeting the template 
            strand of rfp, SpecR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Cleto S, Jensen JV, Wendisch VF, Lu TK
  TITLE     Corynebacterium glutamicum Metabolic Engineering with CRISPR 
            Interference (CRISPRi).
  JOURNAL   ACS Synth Biol. 2016 Feb 16.
  PUBMED    26829286
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Feb 16."
COMMENT     Sequence Label: pAL-rfp_T
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        72..147
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 aatattctga aatgagctgt tgacaattaa tcaggctcgt ataatgaagt tcgtatggaa
       61 ggttccgtta agttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
      121 cttgaaaaag tggcaccgag tcggtgcttt ttatggaatt ggatccgtgg aagacatctg
      181 tcgaagacac cttgtggtcg aagacacctt gtctcttccc agaggcgtgg tcgcatacta
      241 atttgtaaac ggccatgacg aaatagtcgt acggaacaag gagccactag tctagaattc
      301 ggtgaggtta tggcggaggg ttgcgaggtc taggagaaca gaggaagtca tgctttgaag
      361 catataagct gccctgcccc tcaaggtttt cttcaagtga ggttttatct aactgcctaa
      421 cggcagggga accgtatatt gcttacggta tgagacccct taaacgtccg gatagtcacc
      481 gctcttcttt agctccgcga catgcctagc aaccgtggcg cgagagactc ctacctctgc
      541 ccctatttca gcccacgtgg gaactgtccc tgtctggaaa tactgatcgt tcaccatttg
      601 gctaatacgg gacttcgtag atcgtccttg agcctttttc ttacggtgcg tcttttcaag
      661 cttcgacctt tgtgcttgcg catatttgcc ctcggggtct gttttccagc gttgtgcggc
      721 ttt
//
