LOCUS       sequence-140556-        6906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express GFP-nArgBP2-Delta-NSE fusion protein in mammalian cells. 
            nArgBP2-Delta-NSE is the mutant without the neuronal specific exon 
            of nArgBP2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Zhang Q, Gao X, Li C, Feliciano C, Wang D, Zhou D, Mei Y, Monteiro 
            P, Anand M, Itohara S, Dong X, Fu Z, Feng G
  TITLE     Impaired Dendritic Development and Memory in Sorbs2 Knock-Out Mice.
  JOURNAL   J Neurosci. 2016 Feb 17;36(7):2247-60. doi: 
            10.1523/JNEUROSCI.2528-15.2016.
  PUBMED    26888934
REFERENCE   2  (bases 1 to 6906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.2528-15"; journalName: "J Neurosci"; date: 
            "2016-02-17- 17"; volume: "36"; issue: "7"; pages: "2247-60"
COMMENT     Sequence Label: GFP-nArgBP2-Delta-NSE
FEATURES             Location/Qualifiers
     source          1..6906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     RBS             2025..2033
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             3485..3496
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     polyA_signal    3694..3815
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3731..3750)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3785..3804
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3822..4277)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3959..3980)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4171..4190
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4304..4408
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4410..4767
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4410..4601
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4433..4453)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4618..4753
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4802..5596
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4802..5596
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4856..4875)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5466..5485
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5784..5803)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5828..5875
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6204..6792
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6693..6712
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggccggac tcagatctcg agctccatga atacagatag cggtgggtgt
     1381 gctcgcaaac gtgccgccat gtctgttacg ttaacatctg tgaagagagt tcaaagttct
     1441 ccaaacctat tggctgcagg gcgtgagtct cagtctccag attcagcaaa aggttttaga
     1501 agcgttcggc caaacctgca agacaaaaga tcaccgaccc agagccagat cactatcaat
     1561 ggcaactctg gtggcgccgt gagtccagtg agttactatc agaggccatt ctccccttct
     1621 gcatactccc tcccagcctc gctgaactcc agcattatca tgcagcacgg caggtctctt
     1681 gattctgcag agacatattc ccagcatgcc cagtccctgg atggcaccat gggaagctcc
     1741 atcccgctct atagatcctc cgaggaagag aagagggtca cggtcatcaa agccccgcat
     1801 tacccaggga tcggccctgt ggatgagtct ggaatcccca cagccattag gacgactgtc
     1861 gacaggccaa aggactggta caagacaatg ttcaagcaaa tccacatggt acacaagccg
     1921 ggtctgtaca actcgcccta cagtgctcag tcccacccgg ctgcgaagac tcagacctac
     1981 agacctcttt cgaaaagcca ctcagacaat ggcaccgatg cgtttaagga ggtgccctcc
     2041 ccagtgcctc ccccacacgt cccaccacga ccaagagatc agtcttcaac actaaagcat
     2101 gactgggacc ctccagacag aaaggtggac accaggaaat ttcgatcgga gccaaggagt
     2161 atttttgaat atgagcctgg gaagtcgtcc attctgcagc acgaaagacc cccccctctc
     2221 ccaccaactc cgacacctgt tcccagggaa cccagccgga agcctctctc agtgtcaccc
     2281 tcaacagatg gcctgcgtag cccttcccct ccgcccagga gctgtgtccc cgcaccccgt
     2341 ccgagtgccc cagatctctc tcccacccgg acgggtcgca taaatccagc tgacatagat
     2401 ttagaaaatg aaccctggta taaattcttt tcagaactgg agtttggaca cccgcctcct
     2461 aaaaaggctc tggattatgt tcaagatcat tcttccggtg tgtccaacga ggtctccatc
     2521 tatcagtcct ccatagacag aagcctggaa agacccagca gctctgcaag catggccggt
     2581 gactttagga aacggaggaa gagcgaacct gcagtgggcc cactccgggg cttgggggat
     2641 cagagttcaa gcaggaccag ccctggccgg gcggacctcc caggatcaag ttccaccttt
     2701 acgaagtctt tcattagctc ttctccttcc tctccctcga gagcacaaga tcacgagtcc
     2761 cctagaagtt actcatctac tctgactgac ttgggcagaa gtgcatcacg ggaacgaagg
     2821 ggaactccag aaaaagagaa attgcctgca aaagctgtct atgatttcaa agctcagaca
     2881 tctaaggagc tgtcatttaa gaaaggagac actgtctaca tcctcaggaa aattgaccaa
     2941 aactggtatg agggggaaca ccacggcaga gtgggcattt tcccaatctc atacgtagag
     3001 aaactaacac ccccggaaaa agcgcagccc gcaagaccac cacccccagt ccagcccgga
     3061 gagattggag aagccatagc caagtacaac ttcaatgcag acacaaatgt ggaactctcc
     3121 ctgcgaaagg gcgacaggat tattcttctt aaaagagttg accaaaactg gtacgaaggt
     3181 aaaatcccag gaaccaacag acaaggcatc ttccccgtgt cctatgtcga ggttgtcaag
     3241 aggaacgcaa aaggtgctga ggactaccca gaccctcctc tgccccacag ctactccagt
     3301 gacagaattt acaccctaag ctccaataag ccacagcgtc ctgggttctc tcatgaaaac
     3361 attcaaggtg ggggagaacc gtttcaggct ctgtataact atactcctag gaacgaagat
     3421 gagctggaac tcagagaaag tgatgtcgtt gatgtcatgg aaaagtgtga cgacggatgg
     3481 tttgtgggaa cttcaagaag aaccaaattc tttggtactt ttcccggaaa ctatgtcaaa
     3541 aggctgtgag gtaccgcggg cccgggatcc accggatcta gataactgat cataatcagc
     3601 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     3661 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     3721 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     3781 agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat
     3841 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     3901 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     3961 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     4021 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     4081 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     4141 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     4201 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     4261 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4321 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4381 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
     4441 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4501 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4561 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4621 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4681 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     4741 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
     4801 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     4861 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     4921 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     4981 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     5041 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     5101 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     5161 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     5221 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     5281 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
     5341 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     5401 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     5461 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     5521 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     5581 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     5641 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     5701 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     5761 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     5821 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
     5881 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
     5941 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
     6001 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
     6061 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6121 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6181 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6241 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6301 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     6361 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6421 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6481 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6541 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6601 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6661 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6721 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6781 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6841 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     6901 atgcat
//
