LOCUS       sequence-140560-        8764 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for use as a standard for titering experiments 
            when trying to compare vectors with different selection markers or 
            to determine appropriate antibiotic conditions..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8764)
  TITLE     David Root plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRosetta
FEATURES             Location/Qualifiers
     source          1..8764
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..501
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     420..438
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(513..532)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1009..1029
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             1122..1178
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1179..1574
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             1611..1676
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             join(1683..1685,1686..1688,1689..2405)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKS"
     primer_bind     complement(1728..1749)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1989..2008)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2336..2357
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2420..3008
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2473..2493)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2891..2902)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3080..3313
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3391..3512
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3428..3447)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3482..3501
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3552..3687
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3707..3726)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3708..3726)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3736..3753)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3736..3752)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3745..3767)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3894..4349
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3981..4000)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4191..4212
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4375..4479
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4480..4548,4549..5340)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4698..4717)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5511..6099
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6000..6019
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6253..6270
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6387..6408
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6423..6428,6429..6446,6447..6453)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6461..6477
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6466..6488
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6485..6501
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6485..6501
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6520..6540
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6522..6540
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6568..6794
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6795..6975
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7022..7147
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7640..7873
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8058..8102
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8280..8528
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8280..8300
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8451..8470
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    8589..8706
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
       61 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
      121 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
      181 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
      241 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
      301 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
      361 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
      421 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
      481 atcaccgacc tctctcccca gggggatcca ccatgaccga gtacaagccc accgtgcgcc
      541 tcgccacccg cgacgacgtg cccagggccg tccgcaccct cgccgccgct ttcgccgact
      601 accccgccac ccgccacacc gtcgatcccg accgccacat cgagcgggtc accgagctgc
      661 aagaactctt cctcacccgc gtcgggctcg acatcggcaa ggtgtgggtc gctgacgacg
      721 gcgccgcagt ggctgtctgg accacacccg agagcgtcga agctggggcg gtgttcgccg
      781 agatcggccc tcgcatggcc gagttgagcg gttcccggct ggccgctcag caacagatgg
      841 aaggcctcct ggctccacac cggcccaagg agcccgcatg gttcctggcc accgtcggcg
      901 tctctcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
      961 ctgccgagcg cgccggggtg cccgccttcc tggagacctc cgcaccccgc aacctcccct
     1021 tctacgagcg gctcggcttc accgtcaccg ccgacgtgga ggtgcccgaa ggaccacgca
     1081 cctggtgcat gacccgcaag cccggtgccc tgggaagcgg agctactaac ttcagcctgc
     1141 tgaagcaggc tggagacgtg gaggagaacc ctggacctat ggccaagcct ttgtctcaag
     1201 aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag
     1261 actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg
     1321 tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg
     1381 cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga
     1441 gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag
     1501 tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt
     1561 atgtgtggga gggccaggga tctggcgaag ccagacacaa acagaaaatt gtgaaacaga
     1621 ctttgaattt tgaccttctc aagttggcgg gagacgtgga gtccaaccca gggcccgcta
     1681 gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     1741 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     1801 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     1861 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     1921 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     1981 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     2041 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     2101 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     2161 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     2221 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     2281 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     2341 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     2401 cctaacgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     2461 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     2521 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     2581 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     2641 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     2701 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     2761 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     2821 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     2881 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     2941 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     3001 ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     3061 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     3121 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     3181 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     3241 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     3301 aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt
     3361 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     3421 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     3481 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     3541 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     3601 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     3661 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     3721 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     3781 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     3841 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     3901 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     3961 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4021 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4081 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     4141 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4201 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     4261 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4321 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     4381 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4441 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4501 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4561 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     4621 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     4681 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     4741 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     4801 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     4861 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     4921 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4981 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     5041 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5101 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5161 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     5221 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5281 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     5341 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     5401 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5461 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5521 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5581 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5641 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     5701 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5761 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5821 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5881 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5941 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6001 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6061 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6121 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6181 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     6241 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     6301 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     6361 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     6421 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     6481 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     6541 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     6601 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     6661 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     6721 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     6781 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6841 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6901 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6961 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     7021 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     7081 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     7141 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     7201 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     7261 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     7321 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     7381 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     7441 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     7501 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     7561 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     7621 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     7681 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     7741 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     7801 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     7861 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     7921 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     7981 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     8041 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     8101 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     8161 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     8221 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccagag
     8281 agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga
     8341 taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa
     8401 agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata
     8461 tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     8521 gaggtaccgg acagttaacc actttttgaa ttctagatct tgagacaaat ggcagtattc
     8581 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     8641 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     8701 aattttcggg tttattacag ggacagcaga gatccacttt ggcgccggct cgagggggcc
     8761 cggg
//
