LOCUS       sequence-140561-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 2 Destination vector - Position D - Assembly of 4-5 TU's from 
            Level 1-ABCD or ABCDE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pTU2-D-RFP
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(21..64)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    67..88
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    210..231
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(246..251,252..269,270..276)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    284..300
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             318..329
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga
       61 tttctggaat tcgcggccgc ttctagaggt ctcatcgaat ctagagacgc aatacgcaaa
      121 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
      181 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
      241 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
      301 tttcacacat actagagaaa gaggagaaat actagatggc ttcctccgaa gacgttatca
      361 aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa
      421 tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta
      481 ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt
      541 ccaaagctta cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg
      601 aaggtttcaa atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc
      661 aggactcctc cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact
      721 tcccgtccga cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac
      781 gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aa
//
