LOCUS       sequence-140565-       11736 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lenti vector encoding the MS2-P65-HSF1 activator helper complex with
            a 2A Hygro resistance marker NOTE: A version of this plasmid with 
            improved titer is available: Addgene plasmid #89308 lentiMPH v2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11736)
  AUTHORS   Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena 
            C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F
  TITLE     Genome-scale transcriptional activation by an engineered CRISPR-Cas9
            complex.
  JOURNAL   Nature. 2014 Dec 10. doi: 10.1038/nature14136.
  PUBMED    25494202
REFERENCE   2  (bases 1 to 11736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Dec 10. doi: 10.1038/nature14136."
COMMENT     Sequence Label: lenti MS2-P65-HSF1_Hygro
FEATURES             Location/Qualifiers
     source          1..11736
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2687..3865
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2918..3856
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3813..3833
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3872..3881
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3881..4267
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             4322..4342
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4358..4900
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             4925..5296
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             5312..5365
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5369..6388
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    6428..7016
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6481..6501)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6899..6910)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7019..7035)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7020..7036)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7541..7721
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7747..7764)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7753..7977
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8023..8451
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8110..8129)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8320..8341
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8460..8480)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8465..8794
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8645..8780
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8707..8726
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8842..8889
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8908..9282
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9412..9533
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9449..9468)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9503..9522
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9582..9598)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9582..9598)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9595..9617)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9606..9622
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9630..9636,9637..9654,9655..9660))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9675..9696
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9813..9830)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9984..10572)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10064..10083)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10743..11534,11535..11603))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11366..11385
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11604..11708)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagctgca aagatggata aagttttaaa cagagaggaa
     2641 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg
     2701 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     2761 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     2821 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     2881 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     2941 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
     3001 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
     3061 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     3121 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     3181 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     3241 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     3301 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     3361 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     3421 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     3481 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tgaaggacgc ggcgctcggg
     3541 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     3601 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
     3661 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     3721 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     3781 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     3841 ttttcttcca tttcaggtgt cgtgacgtac ggccaccatg gcttcaaact ttactcagtt
     3901 cgtgctcgtg gacaatggtg ggacagggga tgtgacagtg gctccttcta atttcgctaa
     3961 tggggtggca gagtggatca gctccaactc acggagccag gcctacaagg tgacatgcag
     4021 cgtcaggcag tctagtgccc agaagagaaa gtataccatc aaggtggagg tccccaaagt
     4081 ggctacccag acagtgggcg gagtcgaact gcctgtcgcc gcttggaggt cctacctgaa
     4141 catggagctc actatcccaa ttttcgctac caattctgac tgtgaactca tcgtgaaggc
     4201 aatgcagggg ctcctcaaag acggtaatcc tatcccttcc gccatcgccg ctaactcagg
     4261 tatctacagc gctggaggag gtggaagcgg aggaggagga agcggaggag gaggtagcgg
     4321 acctaagaaa aagaggaagg tggcggccgc tggatcccct tcagggcaga tcagcaacca
     4381 ggccctggct ctggccccta gctccgctcc agtgctggcc cagactatgg tgccctctag
     4441 tgctatggtg cctctggccc agccacctgc tccagcccct gtgctgaccc caggaccacc
     4501 ccagtcactg agcgctccag tgcccaagtc tacacaggcc ggcgagggga ctctgagtga
     4561 agctctgctg cacctgcagt tcgacgctga tgaggacctg ggagctctgc tggggaacag
     4621 caccgatccc ggagtgttca cagatctggc ctccgtggac aactctgagt ttcagcagct
     4681 gctgaatcag ggcgtgtcca tgtctcatag tacagccgaa ccaatgctga tggagtaccc
     4741 cgaagccatt acccggctgg tgaccggcag ccagcggccc cccgaccccg ctccaactcc
     4801 cctgggaacc agcggcctgc ctaatgggct gtccggagat gaagacttct caagcatcgc
     4861 tgatatggac tttagtgccc tgctgtcaca gatttcctct agtgggcagg gaggaggtgg
     4921 aagcggcttc agcgtggaca ccagtgccct gctggacctg ttcagcccct cggtgaccgt
     4981 gcccgacatg agcctgcctg accttgacag cagcctggcc agtatccaag agctcctgtc
     5041 tccccaggag ccccccaggc ctcccgaggc agagaacagc agcccggatt cagggaagca
     5101 gctggtgcac tacacagcgc agccgctgtt cctgctggac cccggctccg tggacaccgg
     5161 gagcaacgac ctgccggtgc tgtttgagct gggagagggc tcctacttct ccgaagggga
     5221 cggcttcgcc gaggacccca ccatctccct gctgacaggc tcggagcctc ccaaagccaa
     5281 ggaccccact gtctcctgta caggcagtgg agagggcaga ggaagtctgc taacatgcgg
     5341 tgacgtcgag gagaatcctg gcccaaccat gaaaaagcct gaactcaccg ctacctctgt
     5401 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctccgaggg
     5461 cgaagaatct cgggctttca gcttcgatgt gggagggcgt ggatatgtcc tgcgggtgaa
     5521 tagctgcgcc gatggtttct acaaagatcg ctatgtttat cggcactttg catccgccgc
     5581 tctccctatt cccgaagtgc ttgacattgg ggagttcagc gagagcctga cctattgcat
     5641 ctcccgccgt gcacagggtg tcaccttgca agacctgcct gaaaccgaac tgcccgctgt
     5701 tctccagccc gtcgccgagg ccatggatgc catcgctgcc gccgatctta gccagaccag
     5761 cgggttcggc ccattcggac ctcaaggaat cggtcaatac actacatggc gcgatttcat
     5821 ctgcgctatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag
     5881 tgcctccgtc gcccaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt
     5941 ccggcacctc gtgcacgccg atttcggctc caacaatgtc ctgaccgaca atggccgcat
     6001 aacagccgtc attgactgga gcgaggccat gttcggggat tcccaatacg aggtcgccaa
     6061 catcttcttc tggaggccct ggttggcttg tatggagcag cagacccgct acttcgagcg
     6121 gaggcatccc gagcttgcag gatctcctcg gctccgggct tatatgctcc gcattggtct
     6181 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggctcaggg
     6241 tcgctgcgac gcaatcgtcc ggtccggagc cgggactgtc gggcgtacac aaatcgcccg
     6301 cagaagcgct gccgtctgga ccgatggctg tgtggaagtg ctcgccgata gtggaaacag
     6361 acgccccagc actcgtccta gggcaaagga tctgcagtaa tgagaattcg atatcaagct
     6421 tatcggtaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6481 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     6541 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     6601 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     6661 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     6721 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     6781 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     6841 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     6901 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     6961 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     7021 ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     7081 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     7141 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     7201 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct
     7261 tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg
     7321 gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca
     7381 agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct
     7441 gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc
     7501 atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca
     7561 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7621 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7681 atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct
     7741 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     7801 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     7861 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     7921 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     7981 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     8041 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     8101 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     8161 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     8221 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     8281 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     8341 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     8401 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     8461 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     8521 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     8581 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     8641 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     8701 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     8761 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     8821 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
     8881 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
     8941 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
     9001 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
     9061 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
     9121 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
     9181 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
     9241 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc
     9301 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     9361 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     9421 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     9481 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     9541 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     9601 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     9661 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     9721 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     9781 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     9841 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     9901 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     9961 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    10021 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    10081 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    10141 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    10201 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    10261 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    10321 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    10381 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
    10441 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    10501 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    10561 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    10621 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    10681 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    10741 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    10801 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    10861 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    10921 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    10981 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    11041 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    11101 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    11161 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    11221 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    11281 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    11341 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    11401 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    11461 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    11521 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    11581 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    11641 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    11701 gttccgcgca catttccccg aaaagtgcca cctgac
//
