LOCUS       sequence-140568-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Entry vector"" ORF - eGFP                         "".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-ORF-eGFP
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(22..65)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    68..87
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             114..827
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(144..172)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     767..787
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg
       61 atttctggaa ttcgcggccg cttctagaat ctagagacgc ataggtctca catatgcgta
      121 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
      181 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
      241 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta
      301 ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact
      361 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg
      421 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa
      481 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat
      541 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag
      601 ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc
      661 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca
      721 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga tcacatggtc cttcttgagt
      781 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa tcgaagagac
      841 ccatgccgtc tcattagctg cagtccggca aa
//
