LOCUS       sequence-140570-         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Entry vector"" ORF - eCFP"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-ORF-eCFP
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(11..13,14..16,17..529)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(56..77)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(317..336)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     664..685
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggtctcacat atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
       61 cgagctggac ggcgacgtga acggccacaa gttcagcgtg tccggcgagg gcgagggcga
      121 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
      181 ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc gctaccccga
      241 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
      301 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
      361 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
      421 cctggggcac aagctggagt acaactacat cagccacaac gtctatatca ccgccgacaa
      481 gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt
      541 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
      601 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
      661 tcacatggtc ctgctggagt tcgtgaccgc cgcctaagga tcctcgaaga gacc
//
