LOCUS       sequence-140571-       13462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid for tet-inducible expression of mouse Oct4, Sox2,
            Klf4 and Myc for iPS cell generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13462)
  AUTHORS   Carey BW, Markoulaki S, Hanna J, Saha K, Gao Q, Mitalipova M, 
            Jaenisch R
  TITLE     Reprogramming of murine and human somatic cells using a single 
            polycistronic vector.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62.
  PUBMED    19109433
REFERENCE   2  (bases 1 to 13462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Jan 6. 106(1):157-62."
COMMENT     Sequence Label: TetO-FUW-OSKM
FEATURES             Location/Qualifiers
     source          1..13462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     128..147
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        222..326
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(327..395,396..1187)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(545..564)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1358..1946
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1847..1866
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2100..2117
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2234..2255
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2270..2275,2276..2293,2294..2300)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2308..2324
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2313..2335
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2332..2348
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2332..2348
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    2397..2518
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2408..2427)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2462..2481
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2648..3022)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(3041..3088)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(3136..3465)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3150..3285
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3204..3223)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     3450..3470
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(3479..3907)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3589..3610)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3801..3820
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3953..4177
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4166..4183
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             4209..4389
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     4894..4910
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4895..4911
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4914..5502
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5020..5031
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5429..5449
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6909..6968)
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             complement(8439..8492)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(9471..9527)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(10680..10704)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(10701..10739)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(10772..10790,10791..10813,10814..10832,10833..10855,
                     10856..10874,10875..10897,10898..10916,10917..10939,
                     10940..10958,10959..10981,10982..11000,11001..11023,
                     11024..11042)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     misc_feature    11099..11216
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(11514..11558)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11743..11976
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    12469..12594
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             12641..12821
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(12839..13037)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        13039..13418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ctaaccctaa gcagattctt catgcaattg tcggtcaagc cttgccttgt tgtagcttaa
       61 attttgctcg cgcactactc agcgacctcc aacacacaag cagggagcag atactggctt
      121 aactatgcgg catcagagca gattgtactg agagtgcacc ataggggatc gggagatctc
      181 ccgatccgtc gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      241 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      301 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      361 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      421 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      481 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      541 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      601 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
      661 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
      721 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
      781 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
      841 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
      901 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
      961 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1021 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1081 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1141 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1201 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1261 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1321 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1381 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1441 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1501 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1561 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1621 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     1681 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     1741 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     1801 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     1861 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     1921 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     1981 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2041 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2101 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     2161 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     2221 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     2281 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     2341 gctatgacca tgattacgcc aagctctagc tagaggtcga cggtatacag acatgataag
     2401 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     2461 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttgg
     2521 ggtgggcgaa gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc
     2581 ggaaaacgat tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgta
     2641 gcacgtgtca gtcctgctcc tcggccacga agtgcacgca gttgccggcc gggtcgcgca
     2701 gggcgaactc ccgcccccac ggctgctcgc cgatctcggt catggccggc ccggaggcgt
     2761 cccggaagtt cgtggacacg acctccgacc actcggcgta cagctcgtcc aggccgcgca
     2821 cccacaccca ggccagggtg ttgtccggca ccacctggtc ctggaccgcg ctgatgaaca
     2881 gggtcacgtc gtcccggacc acaccggcga agtcgtcctc cacgaagtcc cgggagaacc
     2941 cgagccggtc ggtccagaac tcgaccgctc cggcgacgtc gcgcgcggtg agcaccggaa
     3001 cggcactggt caacttggcc atggtttagt tcctcacctt gtcgtattat actatgccga
     3061 tatactatgc cgatgattaa ttgtcaacac gtgctgatca gatccgaaaa tggatataca
     3121 agctcccggg agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
     3181 gaatagctca gaggcagagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     3241 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     3301 cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     3361 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
     3421 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acagaattaa
     3481 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     3541 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     3601 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     3661 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     3721 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     3781 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     3841 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     3901 ggcgcgtggg gataccccct agagccccag ctggttcttt ccgcctcaga agccatagag
     3961 cccaccgcat ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc
     4021 cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca
     4081 ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag
     4141 gggcaaacaa cagatggctg gcaactagaa ggcacagtcg aggctgatca gcgggtttaa
     4201 acgggccctg ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc
     4261 agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg
     4321 cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca
     4381 gagagaccca gtacagtccg gatgcagctc tcgggccatg tgatgaaatg ctaggcggct
     4441 gtcaaacctc cactctaata cttctctctc cgggtcatcc atcccatgca ggctcacagg
     4501 gtgtaacaag cgggtgttct ctccttcatt ggcttcttct accttctctt gctcaactgg
     4561 tactagcttg tagcaccatc caaaggtcag tggatatctg atccctggcc ctggtgtgta
     4621 gttctgccaa tcagggaagt agccttgtgt gtggtagatc cacagatcaa ggatatcttg
     4681 tcttcgttgg gagtgaatta gcccttccag tccccccttt tcttttaaaa agtggctaag
     4741 atctacagct gccttgtaag tcattggtct taaaggtacc tgaggtgtga ctggaaaacc
     4801 cacctcctcc tcctcttgtg cttctagcca ggcacaatca gcattggtag ctgctgtatt
     4861 gctacttgtg attgctccat gtttttctag gtctcgaggt cgacggtatc gatgcgggga
     4921 ggcggcccaa agggagatcc gactcgtctg agggcgaagg cgaagacgcg gaagaggccg
     4981 cagagccggc agcaggccgc gggaaggaag gtccgctgga ttgagggccg aagggacgta
     5041 gcagaaggac gtcccgcgca gaatccaggt ggcaacacag gcgagcagcc aaggaaagga
     5101 cgatgatttc cccgacaaca ccacggaatt gtcagtgccc aacagccgag cccctgtcca
     5161 gcagcgggca aggcaggcgg cgatgagttc cgccgtggca atagggaggg ggaaagcgaa
     5221 agtcccggaa aggagctgac aggtggtggc aatgccccaa ccagtggggg ttgcgtcagc
     5281 aaacacagtg cacaccacgc cacgttgcct gacaacgggc cacaactcct cataaagaga
     5341 cagcaaccag gatttataca aggaggagaa aatgaaagcc atacgggaag caatagcatg
     5401 atacaaaggc attaaagcag cgtatccaca tagcgtaaaa ggagcaacat agttaagaat
     5461 accagtcaat ctttcacaaa ttttgtaatc cagaggttga ttatcgataa gcttgatatc
     5521 gaattcggcg cgccttatgc accagagttt cgaagctgtt cgagtttgtg tttcaactgt
     5581 tctcgtcgtt tcctcaataa gtccttttca gaggtgagct tgtgctcgtc tgcttgaatg
     5641 gacaggatgt aggcggtggc ttttttgagg atcactacct tgggggcctt ttcgttgttt
     5701 tccaattcag ggatctggtc acgcagggca aaaaagctgc gcttcagctc gttcctcctc
     5761 tgacgttcca agacgttgtg tgtccgcctc ttgtcgtttt cctccgtgtc tgaggacctg
     5821 gggctggagc acttgcggtt gttgctgatc tgcttcagga ccctgccact gtccaacttg
     5881 gccctcttgg cagctggata gtccttcctt gtggaggggg gtgcggcgta gttgtgctgg
     5941 tgagtggaga cgtggcacct cttgaggacc agtgggctgt gcggaggttt gctgtggcct
     6001 cgggatggag atgagcccga ctccgacctc ttggcagggg tttgcctctt ctccacagac
     6061 accacatcaa tttcttcctc atcttcttgc tcttcttcag agtcgctgct ggtggtgggc
     6121 ggtgtctcct catgcagcac taggggctca gggctggccc gtggggagga ctcggaggac
     6181 agcagcgagt ccgaggaagg agagaaggcc gtggaatcgg acgaggtaca ggatttgggc
     6241 gagctgctgt cgttgagcgg gtagggaaag accactgagg ggtcaatgca ctcggacgcg
     6301 gcggcggtga ggtcctgcag gtacaggctg gaggtggagc agacgctgtg cccgcgggcg
     6361 gggctcaggc tggtgctgtc tttgcgcgca gcctggtagg aggccagctt ctccgagacc
     6421 agcttggcag cggctgagaa accgctccac atacagtcct ggatgatgat gttcttgatg
     6481 aaggtctcgt cgtcaggatc gcagatgaag ctctggttca ccatgtctcc tccaagtaac
     6541 tcggtcatca tctccagctg atcggcggtg gagaagttgc caccgccgcc gtcatcgtct
     6601 tcccttgggg agaaggacgt agcgaccgca acataggatg gagagcagag cccggagcgg
     6661 cggctcgggg acaggggcgg ggtgggaagc agctcgaatt tcttccagat atcctcactg
     6721 ggcgcgggcg gctgcagctc gctctgctgt tgctggtgat agaaattctc ttcctcgtcg
     6781 cagatgaaat agggctgtac ggagtcgtag tcgaggtcat agttcctgtt ggtgaagttc
     6841 acgttgaggg gcatcgtcgt ggctgtctgc ggggtttcca acgcccaaag gaaatccagt
     6901 ggcgcgccgg gacctgggtt gctctcaaca tctccagcca atttcaagag agcataatta
     6961 gtacactggc ccgagccctc gagaaagtgc ctcttcatgt gtaaggcaag gtggtccgac
     7021 ctggaaaagg ccctgtcgca cttctggcac tgaaagggcc ggtgccctgt gtgtttgcgg
     7081 tagtgcctgg tcagttcatc ggagcgggcg aatttccacc cacagccgtc ccagtcacag
     7141 tggtaaggtt tctcgcctgt gtgagttcgc aggtgtgcct tgagatgaga actcttggta
     7201 taggttttgc cacagcctgc atagtcacaa gtgtgggtgg ctgttctttt ccggggccac
     7261 gaccttcttc ccctctttgg cttgggctcc tctggcaggc aggaacccgg tggcatgagc
     7321 tcttgataat ggagagaggg gacttgtgac tgcatctggt ctggcaggaa aggagggtag
     7381 ttgggccccg gatggggatg gaatcctggg ggaagaggca ggccagggtg acagtccctg
     7441 ctgttcagca gttcctcggg actcagtgta ggggtagtcc tggtggggag ctgccgcccc
     7501 agggggaagt cgtgtgtgtt gggccggtgg ccgttgctga gctggggtcc agcgctcaaa
     7561 tgggcctcta gggaccggct gaccgtgcag gacgggaccg cctcttgctt aatcttgggg
     7621 cacatgcgcg gcgggccacc gctgtagggc gccactacca cggggtggct gccgtctggg
     7681 cttcctttgc taacactgat gaccgaaggg ctgctgtact cgctgccagg ggtggtcaga
     7741 gacgccttca gcacaaactt gcccatcagc ccgccacctg gcggctgagg ctgctgtggc
     7801 ggaatgtata ctgggtccaa ctccggccgc aggagctcag ccacgaagcc gcccgagggg
     7861 ctcacgtcat tgatgtccgc caggttgaag ggggccgtgg gaggtggcgc agattctcgg
     7921 ctgtagagga gccctccacc tgtgttgctg gcagccacgc ccgggtcacc cccggcccgg
     7981 atcggatagc tgaagctgca ggtggagggc gcgctggcag ggccgctgct cgctggggaa
     8041 gacgaggatg aagctgacgc cgaggtggtc acggtggcgg ccaccgattc ctggtgggtt
     8101 agcgagttgg aaaggataaa gtctaggtcc aggaggtcgt tgaactcctc ggtctccctc
     8161 cgggctagga gggccgggtt gttactgctg caagctgcac cagctccgcc actctccagg
     8221 tctgtggcca ccgtcgccgc caggtcgtag gggcggccgg gaagtggggg aagtcgcttc
     8281 atgtgagaga gttcctcacg ccaacggtta gtcggggcac ctgctggacg cagtgtcttc
     8341 tcccttcccg ccgggccgga cgcgaacgtg gagaaggacg ggagcagagc gtcgctgaca
     8401 gccatgtcag actcgccagg tggctgcctc atctcgagtg ggccgggatt ttcctccacg
     8461 tccccgcatg ttagaagact tcccctgccc tcgccggagc cgcatgccat gtgcgacagg
     8521 ggcagtgtgc cgttaatggc cgtgccgggc accgggccgc tctggtagtg ctgggccatg
     8581 tgcagtctac tgggcgcagc gggctccggc acctcggcgc cggggaggta catgctgatc
     8641 atgtcccgga ggtccccggc ctggcagggc gccctggagt gggaggaaga ggtaaccacg
     8701 ggggggctgg agctggcctc ggacttgacc acagagccca tggagcccag cgccataccg
     8761 ggggtgccct gctgcgagta ggacatgctg taggtgggcg agccgttcat gtaggtctgc
     8821 gagctggtca tggagttgta ctgcagggcg ctgacgtcgt agcggtgcat cggttgcatc
     8881 tgtgccgcgc cgtgagcgtt gaggcccggg tgctgcgggt agcccagctg ctcctgcatc
     8941 atgctgtagc tgccgttgct ccagccgttc atgtgcgcgt agctgtccat gcgctggttc
     9001 acgcccgcac ccaggccggc gcccacccca accccgctcg ccatgctgtt cccgccgggg
     9061 gccagcaagc ctccgggaag cgtgtactta tccttcttca tgagcgtctt ggttttccgc
     9121 cgcggccggt atttataatc cgggtgctcc ttcatgtgca gagcgcgcag ccgcttggcc
     9181 tcgtcgatga acggccgctt ctcggtctcg gacaaaagtt tccactccgc gcccaggcgc
     9241 ttgctgatct ccgagttgtg catcttgggg ttctcctggg ccatcttacg ccgctgcccc
     9301 cgggaccata ccatgaaggc gttcatgggc ctcttgacgc ggtccgggct gttcttctgg
     9361 ttgccgccgg tcgccgccgc cgtggcgttg cctcctccgc cgccgccccc cgaagcttgc
     9421 tgcgggcccg gcggcttcag ctccgtctcc atcatgttat acatgcatgc aggcccgggg
     9481 ttttcttcaa catctcctgc ttgctttaac agagagaagt tcgtggcgcc gctgccgcta
     9541 gcgtttgaat gcatgggaga gcccagagca gtgacgggaa cagagggaaa ggcctcgccc
     9601 tcaggaaaag ggactgagta gagtgtggtg aagtgggggc ttccatagcc tggggtgcca
     9661 aagtggggac ctgggggcag aggaaaggat acagcccccc ctgggaaagg tgtccctgta
     9721 gcctcatact cttctcgttg ggaatactca atacttgatc ttttgccctt ctggcgccgg
     9781 ttacagaacc atactcgaac cacatccttc tctagcccaa gctgattggc gatgtgagtg
     9841 atctgctgta gggagggctt cgggcacttc agaaacatgg tctccagact ccacctcaca
     9901 cggttctcaa tgctagttcg ctttctcttc cgggcctgca ccagggtctc cgatttgcat
     9961 atctcctgaa ggttctcatt gttgtcggct tcctccaccc acttctccag caggggccgc
    10021 agcttacaca tgttcttaag gctgagctgc aaggcctcga agcgacagat ggtggtctgg
    10081 ctgaacacct ttccaaagag aacgcccagg gtgagcccca cgtcggcctg ggtgtacccc
    10141 aaggtgatcc tcttctgctt cagcagcttg gcaaactgtt ctagctcctt ctgcagggct
    10201 ttcatgtcct gggactcctc gggagttggt tccaccttct ccaacttcac ggcattgggg
    10261 cggtcggcac agggctcaga ggaggttccc tctgagttgc tttccactcg tgctcctgcc
    10321 tggccctcag gctgcaaagt ctccacgcca acttggggga ctaggcccag tccaacctga
    10381 ggtccacagt atgccatccc tccgcagaac tcgtatgcgg gcggacatgg ggagatcccc
    10441 aatacctctg agcctggtcc gattccaggc ccacctggag gcccttggaa gcttagccag
    10501 gttcgaggat ccacccagcc cggctccagc cctgctgacc catcaccccc acctggtggg
    10561 ggtgagaagg cgaagtctga agccaggtgt ccagccatgg cgaattcggg gccgcggagg
    10621 ctggatcggt cccggtgtct tctatggagg tcaaaacagc gtggatggcg tctccaggcg
    10681 atctgacggt tcactaaacg agctctgctt atataggcct cccaccgtac acgcctacct
    10741 cgacccgggt accgagctcg actttcactt ttctctatca ctgataggga gtggtaaact
    10801 cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgactttc acttttctct
    10861 atcactgata gggagtggta aactcgactt tcacttttct ctatcactga tagggagtgg
    10921 taaactcgac tttcactttt ctctatcact gatagggagt ggtaaactcg actttcactt
    10981 ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat cactgatagg
    11041 gagtggtaaa ctcgagatct aaccaaactg gatctctgct gtccctgtaa taaacccgaa
    11101 aattttgaat ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta
    11161 tgtctactat tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaattgt
    11221 ggatgaatac tgccatttgt ctgcagaatt ggcgcacgca gtgccgatcc gttcactaat
    11281 cgaatggatc tgtctctgtc tctctctcca ccttcttctt ctattccttc gggcctgtcg
    11341 ggtcccctcg gggttgggag gtgggtctga aacgataatg gtgaatatcc ctgcctaact
    11401 ctattcacta tagaaagtac agcaaaaact attcttaaac ctaccaagcc tcctactatc
    11461 attatgaata attttatata ccacagccaa tttgttatgt taaaccaatt ccacaaactt
    11521 gcccatttat ctaattccaa taattcttgt tcattctttt cttgctggtt ttgcgattct
    11581 tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca ctccatccag
    11641 gtcgtgtgat tccaaatctg ttccagagat ttattactcc aactagcatt ccaaggcaca
    11701 gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc ccaggagctg ttgatccttt
    11761 aggtatcttt ccacagccag gattcttgcc tggagctgct tgatgcccca gactgtgagt
    11821 tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg ctgcactata
    11881 ccagacaata attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt
    11941 cctgctgctc ccaagaaccc aaggaacaaa gctcctattc ccactgctct tttttctctc
    12001 tgcaccactc ttctctttgc cttggtgggt gctactccta atggttcaat ttttactact
    12061 ttatatttat ataattcact tctccaattg tccctcatat ctcctcctcc aggtctgaag
    12121 atcagcggcc gcttgctgtg cggtggtctt acttttgttt tgctcttcct ctatcttgtc
    12181 taaagcttcc ttggtgtctt ttatctctat cctttgatgc acacaataga gggttgctac
    12241 tgtattatat aatgatctaa gttcttctga tcctgtctga agggatggtt gtagctgtcc
    12301 cagtatttgt ctacagcctt ctgatgtttc taacaggcca ggattaactg cgaatcgttc
    12361 tagctccctg cttgcccata ctatatgttt taatttatat tttttctttc cccctggcct
    12421 taaccgaatt ttttcccatc gcgatctaat tctcccccgc ttaatactga cgctctcgca
    12481 cccatctctc tccttctagc ctccgctagt caaaattttt ggcgtactca ccagtcgccg
    12541 cccctcgcct cttgccgtgc gcgcttcagc aagccgagtc ctgcgtcgag agagctcctc
    12601 tggtttccct ttcgctttca agtccctgtt cgggcgccac tgctagagat tttccacact
    12661 gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact
    12721 acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca
    12781 gagagctccc aggctcagat ctggtctaac cagagagacc cagtacaggc aaaacgcgct
    12841 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt
    12901 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc
    12961 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
    13021 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt
    13081 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
    13141 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
    13201 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg
    13261 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc
    13321 atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt
    13381 aattgattac tattaataac tagtcaataa tcaatgtcaa cgcgtatatc tggcccgtac
    13441 atcgcgaagc agcgcaaaac gc
//
