LOCUS       sequence-140573-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-J23100
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(22..65)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    68..87
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     RBS             115..126
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      160..217
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(241..258)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(492..511)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg
       61 atttctggaa ttcgcggccg cttctagaat ctagagacgc ataggtctca gtacaaagag
      121 gagaaaggac agagacccat gccgtctcat tagctgcagt ccggcaaaaa agggcaaggt
      181 gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc
      241 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
      301 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
      361 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag
      421 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      481 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      541 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
      601 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
      661 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
      721 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
      781 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
      841 ctacactaga aga
//
