LOCUS       sequence-140577-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 - SJM902 promoter (5' CTAT/3' GTAC fusion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-SJM902
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(22..65)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    68..87
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     promoter        112..146
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     primer_bind     complement(220..248)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg
       61 atttctggaa ttcgcggccg cttctagaac gtctcaatct ggtctcacta ttttacagct
      121 agctcagtcc tagggattat gctagcgtac agagaccaga tcttttaact ttaagaagga
      181 gatatacata tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa
      241 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca
      301 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg
      361 ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat
      421 atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact
      481 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat
      541 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt
      601 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa
      661 aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa
      721 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac
      781 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagatcac
      841 atggtccttc ttgagtttgt a
//
