LOCUS       sequence-140585-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 - SJM914 promoter (5' CTAT/3' GTAC fusion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-SJM914
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(23..66)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    69..88
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     promoter        114..147
                     /label=BBa_J23113
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.01"
     primer_bind     complement(221..249)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ataggcggta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
       61 gatttctgga attcgcggcc gcttctagaa cgtctcaatc tggtctcact atttgatggc
      121 tagctcagtc ctagggattg tgctagcgta cagagaccag atcttttaac tttaagaagg
      181 agatatacat atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
      241 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
      301 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
      361 gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca
      421 tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
      481 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga
      541 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
      601 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca
      661 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca
      721 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
      781 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaa
//
