LOCUS       sequence-140592-       12141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human CaV2.2 expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12141)
  AUTHORS   Groen JL, Andrade A, Ritz K, Jalalzadeh H, Haagmans M, Bradley TE, 
            Jongejan A, Verbeek DS, Nurnberg P, Denome S, Hennekam RC, Lipscombe
            D, Baas F, Tijssen MA
  TITLE     CACNA1B mutation is linked to unique myoclonus-dystonia syndrome.
  JOURNAL   Hum Mol Genet. 2015 Feb 15;24(4):987-993. Epub 2014 Oct 8.
  PUBMED    25296916
REFERENCE   2  (bases 1 to 12141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet."; date: "2015-02-15"; volume: "24(4)"; pages: "987-993. Epub 
            2014 Oct 8"
COMMENT     Sequence Label: huCaV2.2 (pSAD442-1)
FEATURES             Location/Qualifiers
     source          1..12141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      899..908
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8006..8047
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             8057..8074
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8097..8114)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8103..8327
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8373..8801
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8460..8479)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8670..8691
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8810..8830)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8815..9144
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8995..9130
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9057..9076
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9192..9239
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9258..9656
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    9814..9935
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9851..9870)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9905..9924
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9984..10000)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9984..10000)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9997..10019)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10008..10024
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10032..10038,10039..10056,10057..10062))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10077..10098
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10215..10232)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10386..10974)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10466..10485)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11145..11936,11937..12005))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11768..11787
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12006..12110)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 caccatggtc cgcttcgggg acgagctggg cggccgctat gggggccccg gcggcggaga
      961 gcgggcccgg ggcggcgggg ccggcggggc ggggggcccg ggtcccgggg ggctgcagcc
     1021 cggccagcgg gtcctctaca agcaatcgat cgcgcagcgc gcgcggacca tggcgctgta
     1081 caaccccatc ccggtcaagc agaactgctt caccgtcaac cgctcgctct tcgtcttcag
     1141 cgaggacaac gtcgtccgca aatacgcgaa gcgcatcacc gagtggcctc cattcgagta
     1201 tatgatcctg gccaccatca tcgccaactg catcgtgctg gccctggagc agcacctccc
     1261 tgatggggac aaaacgccca tgtccgagcg gctggacgac acggagccct atttcatcgg
     1321 gatcttttgc ttcgaggcag ggatcaaaat catcgctctg ggctttgtct tccacaaggg
     1381 ctcttacctg cggaacggct ggaacgtcat ggacttcgtg gtcgtcctca cagggatcct
     1441 tgccacggct ggaactgact tcgacctgcg aacactgagg gctgtgcgtg tgctgaggcc
     1501 cctgaagctg gtgtctggga ttccaagttt gcaggtggtg ctcaagtcca tcatgaaggc
     1561 catggttcca ctcctgcaga ttgggctgct tctcttcttt gccatcctca tgtttgccat
     1621 cattggcctg gagttctaca tgggcaagtt ccacaaggcc tgtttcccca acagcacaga
     1681 tgcggagccc gtgggtgact tcccctgtgg caaggaggcc ccagcccggc tgtgcgaggg
     1741 cgacactgag tgccgggagt actggccagg acccaacttt ggcatcacca actttgacaa
     1801 tatcctgttt gccatcttga cggtgttcca gtgcatcacc atggagggct ggactgacat
     1861 cctctataat acaaacgatg cggccggcaa cacctggaac tggctctact tcatccctct
     1921 catcatcatc ggctccttct tcatgctcaa cctggtgctg ggcgtgctct cgggggagtt
     1981 tgccaaggag cgagagaggg tggagaaccg ccgcgccttc ctgaagctgc gccggcagca
     2041 gcagatcgag cgagagctca acgggtacct ggagtggatc ttcaaggcgg aggaagtcat
     2101 gctggccgag gaggacagga atgcagagga gaagtcccct ttggacgcag tgctgaagag
     2161 agcggccacc aagaagagca gaaatgacct gatccacgca gaggagggag aggaccggtt
     2221 tgcagatctc tgtgctgttg gatccccctt cgcccgcgcc agcctcaaga gcgggaagac
     2281 agagagctcg tcatacttcc ggaggaagga gaagatgttc cggtttttta tccggcgcat
     2341 ggtgaaggct cagagcttct actgggtggt gctgtgcgtg gtggccctga acacactgtg
     2401 tgtggccatg gtgcattaca accagccgcg gcggcttacc acgaccctgt attttgcaga
     2461 gtttgttttc ttgggtctct tcctcacaga gatgtccctg aagatgtatg gcctggggcc
     2521 cagaagctac ttccggtcct ccttcaactg cttcgacttt ggggtcatcg tggggagcgt
     2581 ctttgaagtg gtctgggcgg ccatcaagcc gggaagctcc tttgggatca gtgtgctgcg
     2641 ggccctccgc ctgctgagga tcttcaaagt cacgaagtac tggagctccc tgcggaacct
     2701 ggtggtgtcc ctgctgaact ccatgaagtc catcatcagc ctgctcttct tgctcttcct
     2761 gttcattgtg gtcttcgccc tgctggggat gcagctgttt gggggacagt tcaacttcca
     2821 ggatgagact cccacaacca acttcgacac cttccctgcc gccatcctca ctgtcttcca
     2881 gatcctgacg ggagaggact ggaatgcagt gatgtatcac gggatcgaat cgcaaggcgg
     2941 cgtcagcaaa ggcatgttct cgtcctttta cttcattgtc ctgacactgt tcggaaacta
     3001 cactctgctg aatgtctttc tggccatcgc tgtggacaac ctggccaacg cccaagagct
     3061 gaccaaggat gaagaggaga tggaagaagc agccaatcag aagcttgctc tgcaaaaggc
     3121 caaagaagtg gctgaagtca gccccatgtc tgccgcgaac atctccatcg ccgcttttgt
     3181 aaagcaagct cgaggtactg tatctcgcag ctcatctgtc tccagcgtga actcaccgca
     3241 gcagaactcg gccaaggcgc gctcggtgtg ggagcagcgg gccagccagc tacggctgca
     3301 gaacctgcgg gccagctgcg aggcgctgta cagcgagatg gaccccgagg agcggctgcg
     3361 cttcgccact acgcgccacc tgcggcccga catgaagacg cacctggacc ggccgctggt
     3421 ggtggagctg ggccgcgacg gcgcgcgggg gcccgtggga ggcaaagccc gacctgaggc
     3481 tgcggaggcc cccgagggcg tcgaccctcc gcgcaggcac caccggcacc gcgacaagga
     3541 caagaccccc gcggcggggg accaggaccg agcagaggcc ccgaaggcgg agagcgggga
     3601 gcccggtgcc cgggaggagc ggccgcggcc gcaccgcagc cacagcaagg aggccgcggg
     3661 gcccccggag gcgcggagcg agcgcggccg aggcccaggc cccgagggcg gccggcggca
     3721 ccaccggcgc ggctccccgg aggaggcggc cgagcgggag ccccgacgcc accgcgcgca
     3781 ccggcaccag gatccgagca aggagtgcgc cggcgccaag ggcgagcggc gcgcgcggca
     3841 ccgcggcggc ccccgagcgg ggccccggga ggcggagagc ggggaggagc cggcgcggcg
     3901 gcaccgggcc cggcacaagg cgcagcctgc tcacgaggct gtggagaagg agaccacgga
     3961 gaaggaggcc acggagaagg aggctgagat agtggaagcc gacaaggaaa aggagctccg
     4021 gaaccaccag ccccgggagc cacactgtga cctggagacc agtgggactg tgactgtggg
     4081 tcccatgcac acactgccca gcacctgtct ccagaaggtg gaggaacagc cagaggatgc
     4141 agacaatcag cggaacgtca ctcgcatggg cagtcagccc ccagacccga acactattgt
     4201 acatatccca gtgatgctga cgggccctct tggggaagcc acggtcgttc ccagtggtaa
     4261 cgtggacctg gaaagccaag cagaggggaa gaaggaggtg gaagcggatg acgtgatgag
     4321 gagcggcccc cggcctatcg tcccatacag ctccatgttc tgtttaagcc ccaccaacct
     4381 gctccgccgc ttctgccact acatcgtgac catgaggtac ttcgaggtgg tcattctcgt
     4441 ggtcatcgcc ttgagcagca tcgccctggc tgctgaggac ccagtgcgca cagactcgcc
     4501 caggaacaac gctctgaaat acctggatta cattttcact ggtgtcttta cctttgagat
     4561 ggtgataaag atgatcgact tgggactgct gcttcaccct ggagcctatt tccgggactt
     4621 gtggaacatt ctggacttca ttgtggtcag cggcgccctg gtggcgtttg ctttctcagg
     4681 atccaaaggg aaagacatca ataccatcaa gtctctgaga gtccttcgtg tcctgcggcc
     4741 cctcaagacc atcaaacggc tgcccaagct caaggctgtg tttgattgtg tggtgaactc
     4801 cctgaagaat gtcctcaaca tcttgattgt ctacatgctc ttcatgttca tatttgccgt
     4861 cattgcggtg cagctcttca aagggaagtt tttctactgc acagatgaat ccaaggagct
     4921 ggagagggac tgcaggggtc agtatttgga ttatgagaag gaggaagtgg aagctcagcc
     4981 caggcagtgg aagaaatacg actttcacta cgacaatgtg ctctgggctc tgctgacgct
     5041 gttcacagtg tccacgggag aaggctggcc catggtgctg aaacactccg tggatgccac
     5101 ctatgaggag cagggtccaa gccctgggta ccgcatggag ctgtccatct tctacgtggt
     5161 ctactttgtg gtctttccct tcttcttcgt caacatcttt gtggctttga tcatcatcac
     5221 cttccaggag cagggggaca aggtgatgtc tgaatgcagc ctggagaaga acgagagggc
     5281 ttgcattgac ttcgccatca gcgccaaacc cctgacacgg tacatgcccc aaaaccggca
     5341 gtcgttccag tataagacgt ggacatttgt ggtctccccg ccctttgaat acttcatcat
     5401 ggccatgata gccctcaaca ctgtggtgct gatgatgaag ttctatgatg caccctatga
     5461 gtacgagctg atgctgaaat gcctgaacat cgtgttcaca tccatgttct ccatggaatg
     5521 cgtgctgaag atcatcgcct ttggggtgct gaactatttc agagatgcct ggaatgtctt
     5581 tgactttgtc actgtgttgg gaagtattac tgatatttta gtaacagaga ttgcggaaac
     5641 gaacaatttc atcaacctca gcttcctccg cctctttcga gctgcgcggc tgatcaagct
     5701 gctccgccag ggctacacca tccgcatcct gctgtggacc tttgtccagt ccttcaaggc
     5761 cctgccctac gtgtgtctgc tcattgccat gctgttcttc atctacgcca tcatcggcat
     5821 gcaggtgttt gggaatattg ccctggatga tgacaccagc atcaaccgcc acaacaactt
     5881 ccggacgttt ttgcaagccc tgatgctgct gttcaggagc gccacggggg aggcctggca
     5941 cgagatcatg ctgtcctgcc tgagcaacca ggcctgtgat gagcaggcca atgccaccga
     6001 gtgtggaagt gattttgcct acttctactt cgtctccttc atcttcctgt gctcctttct
     6061 gatgttgaac ctctttgtgg ctgtgatcat ggacaatttt gagtacctca cgcgggactc
     6121 ttccatccta ggtcctcacc acttggatga gttcatccgg gtctgggctg aatacgaccc
     6181 ggctgcgtgt gggcgcatca gttacaatga catgtttgag atgctgaaac acatgtcccc
     6241 gcctctgggg ctggggaaga aatgccctgc tcgagttgct tacaagcgcc tggttcgcat
     6301 gaacatgccc atctccaacg aggacatgac tgttcacttc acgtccacgc tgatggccct
     6361 catccggacg gcactggaga tcaagctggc cccagctggg acaaagcagc atcagtgtga
     6421 cgcggagttg aggaaggaga tttccgttgt gtgggccaat ctgccccaga agactttgga
     6481 cttgctggta ccaccccata agcctgatga gatgacagtg gggaaggttt atgcagctct
     6541 gatgatattc gacttctaca agcagaacaa aaccaccaga gaccagatgc agcaggctcc
     6601 tggaggcctc tcccagatgg gtcctgtgtc cctgttccac cctctgaagg ccaccctgga
     6661 gcagacacag ccggctgtgc tccgaggagc ccgggttttc cttcgacaga agagttccac
     6721 ctccctcagc aatggcgggg ccatacaaaa ccaagagagt ggcatcaaag agtctgtctc
     6781 ctggggcact caaaggaccc aggatgcacc ccatgaggcc aggccacccc tggagcgtgg
     6841 ccactccaca gagatccctg tggggcggtc aggagcactg gctgtggacg ttcagatgca
     6901 gagcataacc cggaggggcc ctgatgggga gccccagcct gggctggaga gccagggtcg
     6961 agcggcctcc atgccccgcc ttgcggccga gactcagccc gtcacagatg ccagccccat
     7021 gaagcgctcc atctccacgc tggcccagcg gccccgtggg actcatcttt gcagcaccac
     7081 cccggaccgc ccacccccta gccaggcgtc gtcgcaccac caccaccacc gctgccaccg
     7141 ccgcagggac aggaagcaga ggtccctgga gaaggggccc agcctgtctg ccgatatgga
     7201 tggcgcacca agcagtgctg tggggccggg gctgcccccg ggagaggggc ctacaggctg
     7261 ccggcgggaa cgagagcgcc ggcaggagcg gggccggtcc caggagcgga ggcagccctc
     7321 atcctcctcc tcggagaagc agcgcttcta ctcctgcgac cgctttgggg gccgtgagcc
     7381 cccgaagccc aagccctccc tcagcagcca cccaacgtcg ccaacagctg gccaggagcc
     7441 gggaccccac ccacagggca gtggttccgt gaatgggagc cccttgctgt caacatctgg
     7501 tgctagcacc cccggccgcg gtgggcggag gcagctcccc cagacgcccc tgactccccg
     7561 ccccagcatc acctacaaga cggccaactc ctcacccatc cacttcgccg gggctcagac
     7621 cagcctccct gccttctccc caggccggct cagccgtggg ctttccgaac acaacgccct
     7681 gctgcagaga gaccccctca gccagcccct ggcccctggc tctcgaattg gctctgaccc
     7741 ttacctgggg cagcgtctgg acagtgaggc ctctgtccac gccctgcctg aggacacgct
     7801 cactttcgag gaggctgtgg ccaccaactc gggccgctcc tccaggactt cctacgtgtc
     7861 ctccctgacc tcccagtctc accctctccg ccgcgtgccc aacggttacc actgcaccct
     7921 gggactcagc tcgggtggcc gagcacggca cagctaccac caccctgacc aagaccactg
     7981 gtgctagtct agagggccct tcgaaggtaa gcctatccct aaccctctcc tcggtctcga
     8041 ttctacgcgt accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc
     8101 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     8161 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     8221 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     8281 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     8341 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     8401 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     8461 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     8521 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     8581 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     8641 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     8701 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     8761 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     8821 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     8881 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     8941 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     9001 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     9061 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     9121 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     9181 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
     9241 aggtgaggaa ctaaaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa
     9301 gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag
     9361 ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac
     9421 cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt
     9481 gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac
     9541 aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc
     9601 cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaagcac
     9661 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
     9721 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     9781 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     9841 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     9901 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     9961 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
    10021 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
    10081 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
    10141 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
    10201 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
    10261 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
    10321 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    10381 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    10441 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    10501 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    10561 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    10621 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    10681 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    10741 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    10801 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
    10861 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    10921 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    10981 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    11041 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    11101 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    11161 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    11221 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    11281 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    11341 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    11401 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    11461 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    11521 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    11581 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    11641 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    11701 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    11761 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    11821 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    11881 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    11941 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    12001 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    12061 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    12121 cgaaaagtgc cacctgacgt c
//
