LOCUS       sequence-140600-         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Packaging plasmid for producing MuLV retroviral particles. Use in 
            conjunction with an envelope plasmid such as pCMV-VSV-G..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Stewart SA, Dykxhoorn DM, Palliser D, Mizuno H, Yu EY, An DS, 
            Sabatini DM, Chen IS, Hahn WC, Sharp PA, Weinberg RA, Novina CD
  TITLE     Lentivirus-delivered stable gene silencing by RNAi in primary cells.
  JOURNAL   RNA 2003 Apr;9(4):493-501.
  PUBMED    12649500
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA"; date:
            "2003-04"; volume: "9(4)"; pages: "493-501"
COMMENT     Sequence Label: pUMVC
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    113..487
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(159..175)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
ORIGIN
        1 ggtaccgggc ccacactgac ccctctgagc atcagaagaa ttaaggggat tagggggcct
       61 cgcgggttaa ccttattttt aaggggtttt gagagcgttg aacgcgccat gtcagtctag
      121 aggatggtcc acccccgggg tcggcagcct tcacgtgggc ggcgtgtatc caagctgcga
      181 tgccgtctac tttgagggcg gtgggggtgg tcagcaggac tgggtaaggt cctttccagc
      241 gaggttctag gttcttagtc tggtgtcggc ggacccacac tgtgtcgccg actcggtaag
      301 ggtgaggtac caccggtcgg tccagttgtt cttggtaggc tgccgccaga ggtctccaga
      361 cttcgtgctg gactaagtag agagcctgta agtgagcttg gagagagggg ctgttagtaa
      421 ctcttgtcat gtcagggtca gggaagttta caaggggcgg gggtgcccca tataagatct
      481 catatggggt gaggccatgg gggcccggcg tgttgcgggc tcggtacagg gctaagggga
      541 gtaggagcac ccagtctcta gagccagttg caagcgttaa tttagttaaa gtctccttga
      601 tggttctatt cattctttct acctggcctg agctttgggg tctgtatgca caatgtaatt
      661 tccaatcaat ccccaacaga tcggccactg tctgactcac cttggagacg aaggcaggcc
      721 cattgtcagt tcccaatacc tgaggcatgc cgaacctggg gaagatctcc tctagtagct
      781 tcttggttac gaccttggcg gtttctttct tggttgggaa ggcttctatc cagccagaaa
      841 aggtatctat aaaaactaga agatatttat agccatacaa tccgggcttt atctcggtga
      901 aatcgatctc ccaatgagtg ccgggccgat gcccgcggac cctagttccc tgtttaacgg
      961 cagacttgct ggcgttgact tgtgcac
//
