LOCUS       sequence-140601-        1002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter gene with five ATF6 sites that is highly sensitive to ER 
            stress.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Wang Y, Shen J, Arenzana N, Tirasophon W, Kaufman RJ, Prywes R
  TITLE     Activation of ATF6 and an ATF6 DNA binding site by the endoplasmic 
            reticulum stress response.
  JOURNAL   J Biol Chem. 2000 Sep 1. 275(35):27013-20.
  PUBMED    10856300
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2000 Sep 1. 275(35):27013-20."
COMMENT     Sequence Label: p5xATF6-GL3
FEATURES             Location/Qualifiers
     source          1..1002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    82..130
                     /note="synthetic polyadenylation signal"
     misc_feature    144..235
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     184..203
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     primer_bind     complement(643..661)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ggcctcttcg ctattacgcc agcccaagct accatgataa gtaagtaata ttaaggtacg
       61 ggaggtactt ggagcggccg caataaaata tctttatttt cattacatct gtgtgttggt
      121 tttttgtgtg aatcgatagt actaacatac gctctccatc aaaacaaaac gaaacaaaac
      181 aaactagcaa aataggctgt ccccagtgca agtgcaggtg ccagaacatt tctctatcga
      241 taggtaccga gctcttacgc gtgctagccc gggctcgacc tgcagcccaa gcttgctcga
      301 gacaggtgct gacgtggcga ttcctcgaga caggtgctga cgtggcgatt cctcgagaca
      361 ggtgctgacg tggcgattcc tcgagacagg tgctgacgtg gcgattcctc gagacaggtg
      421 ctgacgtggc gattcctcga gatctgcact cattcataaa acgcttgtta taaaagcagt
      481 ggctgcggcg cctcgtactc caaccgcatc tgcagcgagc aactgagaag ccaagactga
      541 gccggcggcc tctagagtcg actctagagg atctccatgg aagacgccaa aaacataaag
      601 aaaggcccgg cgccattcta tccgctggaa gatggaaccg ctggagagca actgcataag
      661 gctatgaaga gatacgccct ggttcctgga acaattgctt ttacagatgc acatatcgag
      721 gtggacatca cttacgctga gtacttcgaa atgtccgttc ggttggcaga agctatgaaa
      781 cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc
      841 tttatgccgg tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc gaacgacatt
      901 tataatgaac gtgaattgct caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt
      961 tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa ag
//
