LOCUS       sequence-140603-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FlAsH-BRET reporter to monitor conformational changes in Arrestin3. 
            Insertion of FlAsH motif (CCPGCC) following amino acid residue 225 
            of Arrestin3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Lee MH, Appleton KM, Strungs EG, Kwon JY, Morinelli TA, Peterson YK,
            Laporte SA, Luttrell LM
  TITLE     The conformational signature of beta-arrestin2 predicts its 
            trafficking and signalling functions.
  JOURNAL   Nature. 2016 Mar 31;531(7596):665-8. doi: 10.1038/nature17154. Epub 
            2016 Mar 23.
  PUBMED    27007854
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17154"; journalName: "Nature"; date: "2016-03-31- 
            31"; volume: "531"; issue: "7596"; pages: "665-8"
COMMENT     Sequence Label: Rlucrarr3-Flash4
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    550..671
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(587..606)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     641..660
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(815..836)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taaccccgct gctcagtgac aaccgagaga agcgtggcct tgcccttgat gggcaactca
       61 agcacgaaga caccaacctg gcttccagca ccattgtgaa ggagggagcc aacaaggagg
      121 tgctgggaat cctagtatcc tacagggtca aggtgaagct ggtggtgtct cgaggcgggg
      181 atgtctccgt ggagctacct ttcgtcctaa tgcaccccaa gccccacgac cacatcaccc
      241 ttccccgacc ccagtcagcc ccccgggaaa tagacatccc tgtggatacc aacctcattg
      301 aattcgatac caactatgcc acagacgacg acatcgtgtt tgaggacttt gcgaggcttc
      361 ggctgaaggg gatgaaggat gacgactgtg atgaccagtt ctgctagtct agagggcccg
      421 ggatccaccg gatctagata actgatcata atcagccata ccacatttgt agaggtttta
      481 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
      541 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
      601 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
      661 aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
      721 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
      781 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
      841 aaagaacgtg gactccaacg tcaaagggcg aaaaaa
//
