LOCUS       sequence-140606-         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BAC targeting cassette for pTARBAC2 with kanaR selection marker and 
            lens-specific transgenesis marker Cerulean.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Fuentes F, Reynolds E, Lewellis SW, Venkiteswaran G, Knaut H
  TITLE     A Plasmid Set for Efficient Bacterial Artificial Chromosome (BAC) 
            Transgenesis in Zebrafish.
  JOURNAL   G3 (Bethesda). 2016 Jan 27. pii: g3.115.026344. doi: 
            10.1534/g3.115.026344.
  PUBMED    26818072
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2016 Jan 27. pii: g3.115.026344. doi: 
            10.1534/g3.115.026344."
COMMENT     Sequence Label: pBS-TarHom-Tol2-kanaR-cryaa-CFP
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(132..845,846..848,849..851))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(177..198)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     526..545
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     785..806
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      848..857
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
       61 ggttttttaa agcaagtaaa acctctacaa atatggtatg gctgattatg atctagagtc
      121 gcggccggcc gctacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg
      181 aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag cttggactgg
      241 gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc
      301 tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttg
      361 gccttgatgc cgttcttctg cttgtcggcg gtgatataga cgttgtcgct gatggcgttg
      421 tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg
      481 atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg
      541 tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag
      601 aagtcgtgct gcttcatgtg gtcggggtag cgggcgaagc actgcacgcc ccaggtcagg
      661 gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg
      721 gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg
      781 tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc
      841 ttgctcacca tggtggcgac cggtggatcc acagtacaaa gatccccaaa tgatgtgtgc
      901 cggccttagt ggaggcagac ccctggtta
//
