LOCUS       sequence-140610-         651 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression of mRIPK3 mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 651)
  TITLE     Francis Chan lab plasmids
REFERENCE   2  (bases 1 to 651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HA-mRIPK3 D143N-Flag/cHA-pTRIPZ vector
FEATURES             Location/Qualifiers
     source          1..651
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(98..124)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(134..157)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(255..266)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 gctcgctgcg cccttcgtct gacgtggcag cgcgtcgccg tgcggagggg ggcgcccgcg
       61 ggaggcgcca aaacccggcg cggaggccac gcgtttaagc gtaatctgga acatcgtatg
      121 ggtactcgag ctacttgtca tcgtcgtcct tgtagtcctt gtggaagggc tgccagcccc
      181 tacccctgcc gaactgtgct tggtcatact tggccgaact tgaggcagta gttcttggtg
      241 gtgctaccaa ggagttgtag ttcccaatct gcacttcaga acagttgttg aagacgagag
      301 ccggtggccc tgtcattgga ttcggtgggg tccagggata ccaaggagtg ccgtgtcttc
      361 catctccctg attcctttgg gggtgaggac cgggagtctc agtaaagact ggcccaggtg
      421 ttgtgcctct gaagggtaaa gtatgtggaa tctgaggagt gccagccacg gggtcagaag
      481 atgtccttgc tggtgtggca ggcccaactg atgtgtcctg tgcttgcctc tcaggacatt
      541 ttccaggaac tggtccggag ggttcctcca aatgcaggcg gtccagcatt ttagaaacca
      601 tggtttccct cgggcaatcc atttctgtgc ctctttggct tggctctctg g
//
