LOCUS       sequence-140615-         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RBS_M5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-TL7
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(36..93)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(121..148)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(164..235)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(291..311)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tcagtgagcg aggaagcctg cataacgcga agtaatcttt tcggttttaa agaaaaaggg
       61 cagggtggtg acaccttgcc cttttttgcc ggactgcagc gtctctggca aacatataaa
      121 cgcagaaagg cccacccgaa ggtgagccag tgtgactcta gtagagagcg ttcaccgaca
      181 aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc
      241 tggtcgatta tttgtatagt tcatccatgc catgtgtaat cccagcagct gttacaaact
      301 caagaaggac catgtgatct ctcttttcgt tgggatcttt cgaaagggca gattgtgtgg
      361 acaggtaatg gttgtctggt aaaaggacag ggccatcgcc aattggagta ttttgttgat
      421 aatggtctgc tagttgaacg cttccatctt caatgttgtg tctaattttg aagttaactt
      481 tgattccatt cttttgtttg tctgccatga tgtatacatt gtgtgagtta tagttgtatt
      541 ccaatttgtg tccaagaatg tttccatctt ctttaaaatc aatacctttt aactcgattc
      601 tattaacaag ggtatcacct tcaaacttga cttcagcacg tgtcttgtag ttcccgtcat
      661 ctttgaaaaa tatagttctt tcctgtacat aaccttcggg catggcactc ttgaaa
//
