LOCUS       sequence-140619-         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For recombinant expression of human LC3A/MAP1LC3A in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Ma P, Schwarten M, Schneider L, Boeske A, Henke N, Lisak D, Weber S,
            Mohrluder J, Stoldt M, Strodel B, Methner A, Hoffmann S, Weiergraber
            OH, Willbold D
  TITLE     Interaction of Bcl-2 with the autophagy-related GABAA 
            receptor-associated protein (GABARAP): biophysical characterization 
            and functional implications.
  JOURNAL   J Biol Chem. 2013 Dec 27;288(52):37204-15. doi: 
            10.1074/jbc.M113.528067. Epub 2013 Nov 15.
  PUBMED    24240096
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.528067"; journalName: "J Biol Chem"; date: 
            "2013-12-27- 27"; volume: "288"; issue: "52"; pages: "37204-15"
COMMENT     Sequence Label: pGex-4T-2_LC3A
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             6..368
                     /codon_start=1
                     /gene="MAP1LC3A"
                     /product="human LC3 protein"
                     /label=LC3A
                     /note="Atg8 family member found on autophagosomes"
                     /translation="MPSDRPFKQRRSFADRCKEVQQIRDQHPSKIPVIIERYKGEKQLP
                     VLDKTKFLVPDHVNMSELVKIIRRRLQLNPTQAFFLLVNQHSMVSVSTPIADIYEQEKD
                     EDGFLYMVYASQETFGF"
     RBS             56..64
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(432..454)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(599..617)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gatccatgcc ctcagaccgg cctttcaagc agcggcggag cttcgccgac cgctgtaagg
       61 aggtacagca gatccgcgac cagcacccca gcaaaatccc ggtgatcatc gagcgctaca
      121 agggtgagaa gcagctgccc gtcctggaca agaccaagtt tttggtcccg gaccatgtca
      181 acatgagcga gttggtcaag atcatccggc gccgcctgca gctgaacccc acgcaggcct
      241 tcttcctgct ggtgaaccag cacagcatgg tgagtgtgtc cacgcccatc gcggacatct
      301 acgagcagga gaaagacgag gacggcttcc tctatatggt ctacgcctcc caggaaacct
      361 tcggcttcta agcggccgca tcgtgactga ctgacgatct gcctcgcgcg tttcggtgat
      421 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
      481 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
      541 gcagccatga cccagtcacg tagcgatagc ggagtgtata attcttgaag acgaaagggc
      601 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
      661 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
      721 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaa
//
