LOCUS       sequence-140622-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  L3S1P51 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-L3S1P51
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(36..93)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    209..228
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      231..274
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(278..296)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(524..543)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tcagtgagcg aggaagcctg cataacgcga agtaatcttt tcggttttaa agaaaaaggg
       61 cagggtggtg acaccttgcc cttttttgcc ggactgcagc taatgagacg gcatgcggtc
      121 tctaacattt tgttatcaat aaaaaaggcc ccccgatttg ggaggccttt tttttttttt
      181 cgatgagacc catatgcgtc tctagattct agaagcggcc gcgaattcca gaaatcatcc
      241 ttagcgaaag ctaaggattt tttttatctg aaattctgcc tcgtgatacg cctattttta
      301 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcactcg agttgatcgg
      361 gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt
      421 tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
      481 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
      541 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
      601 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
      661 tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
      721 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
      781 ataccacgac gatttccggc agtttctaca catatattcg ca
//
