LOCUS       sequence-140623-         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  L3S1P32 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-L3S1P32
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(18..75)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    191..210
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      213..256
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(260..278)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(506..525)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tgcataacgc gaagtaatct tttcggtttt aaagaaaaag ggcagggtgg tgacaccttg
       61 cccttttttg ccggactgca gctaatgaga cggcatgcgg tctctaacat tttgttgaaa
      121 aataaaaagg ccccccgatt tgggaggcct tattgttcgt ctcgatgaga cccatatgcg
      181 tctctagatt ctagaagcgg ccgcgaattc cagaaatcat ccttagcgaa agctaaggat
      241 tttttttatc tgaaattctg cctcgtgata cgcctatttt tataggttaa tgtcatgata
      301 ataatggttt cttagacgtc aggtggcact cgagttgatc gggcacgtaa gaggttccaa
      361 ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc
      421 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
      481 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
      541 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
      601 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaatttcg
      661 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
      721 tttccatgag caaactgaaa cgttttcatc gctctggagt ga
//
