LOCUS       sequence-140624-         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For recombinant expression of human LC3B/MAP1LC3B in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Ma P, Schwarten M, Schneider L, Boeske A, Henke N, Lisak D, Weber S,
            Mohrluder J, Stoldt M, Strodel B, Methner A, Hoffmann S, Weiergraber
            OH, Willbold D
  TITLE     Interaction of Bcl-2 with the autophagy-related GABAA 
            receptor-associated protein (GABARAP): biophysical characterization 
            and functional implications.
  JOURNAL   J Biol Chem. 2013 Dec 27;288(52):37204-15. doi: 
            10.1074/jbc.M113.528067. Epub 2013 Nov 15.
  PUBMED    24240096
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.528067"; journalName: "J Biol Chem"; date: 
            "2013-12-27- 27"; volume: "288"; issue: "52"; pages: "37204-15"
COMMENT     Sequence Label: pET-15b_LC3B
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             60..77
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             78..452
                     /codon_start=1
                     /gene="MAP1LC3B"
                     /product="human LC3 protein"
                     /label=LC3B
                     /note="Atg8 family member found on autophagosomes"
                     /translation="MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLP
                     VLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKD
                     EDGFLYMVYASQETFGMKLSV"
     primer_bind     complement(520..538)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      534..581
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac agcagcggcc
       61 tggtgccgcg cggatccatg ccgtcggaga agaccttcaa gcagcgccgc accttcgaac
      121 aaagagtaga agatgtccga cttattcgag agcagcatcc aaccaaaatc ccggtgataa
      181 tagaacgata caagggtgag aagcagcttc ctgttctgga taaaacaaag ttccttgtac
      241 ctgaccatgt caacatgagt gagctcatca agataattag aaggcgctta cagctcaatg
      301 ctaatcaggc cttcttcctg ttggtgaacg gacacagcat ggtcagcgtc tccacaccaa
      361 tctcagaggt gtatgagagt gagaaagatg aagatggatt cctgtacatg gtctatgcct
      421 cccaggagac gttcgggatg aaattgtcag tgtaagcggc gctcgaggat gatccggctg
      481 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
      541 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
      601 ccggatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc
      661 cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg
      721 actgcgttag caatttaact gtgataaact accgcattaa a
//
