LOCUS       sequence-140625-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  L3S1P11 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-L3S1P11
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(36..93)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    207..226
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      229..272
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(276..294)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(522..541)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tcagtgagcg aggaagcctg cataacgcga agtaatcttt tcggttttaa agaaaaaggg
       61 cagggtggtg acaccttgcc cttttttgcc ggactgcagc taatgagacg gcatgcggtc
      121 tctaacattt tgtatcaata aaaaaggccc ccccgaaggg aggccttatt gttcgtctcg
      181 atgagaccca tatgcgtctc tagattctag aagcggccgc gaattccaga aatcatcctt
      241 agcgaaagct aaggattttt tttatctgaa attctgcctc gtgatacgcc tatttttata
      301 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcactcgag ttgatcgggc
      361 acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg
      421 agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata
      481 ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg
      541 ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa
      601 agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg
      661 ctcatccgga atttcgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc
      721 acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat
      781 accacgacga tttccggcag tttctacaca tatattcgca
//
