LOCUS       sequence-140627-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  L2U8H11 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-L2U8H11
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(36..93)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    189..208
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      211..254
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(258..276)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(504..523)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tcagtgagcg aggaagcctg cataacgcga agtaatcttt tcggttttaa agaaaaaggg
       61 cagggtggtg acaccttgcc cttttttgcc ggactgcagc taatgagacg gcatgcggtc
      121 tctaacaata caacaatagc gtgcgtggtt agcacgctat cgatgagacc catatgcgtc
      181 tctagattct agaagcggcc gcgaattcca gaaatcatcc ttagcgaaag ctaaggattt
      241 tttttatctg aaattctgcc tcgtgatacg cctattttta taggttaatg tcatgataat
      301 aatggtttct tagacgtcag gtggcactcg agttgatcgg gcacgtaaga ggttccaact
      361 ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag
      421 gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
      481 aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc
      541 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
      601 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gaatttcgta
      661 tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt
      721 tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc
      781 agtttctaca catatattcg caagatgtgg cgtgttacgg tgaa
//
