LOCUS       sequence-140631-         718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  L2U2H09 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-L2U2H09
FEATURES             Location/Qualifiers
     source          1..718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(36..93)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    186..205
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      208..251
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(255..273)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(501..520)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tcagtgagcg aggaagcctg cataacgcga agtaatcttt tcggttttaa agaaaaaggg
       61 cagggtggtg acaccttgcc cttttttgcc ggactgcagc taatgagacg gcatgcggtc
      121 tctaacatac aaaaaaacgg cccttgcgag ggccgttcga tgagacccat atgcgtctct
      181 agattctaga agcggccgcg aattccagaa atcatcctta gcgaaagcta aggatttttt
      241 ttatctgaaa ttctgcctcg tgatacgcct atttttatag gttaatgtca tgataataat
      301 ggtttcttag acgtcaggtg gcactcgagt tgatcgggca cgtaagaggt tccaactttc
      361 accataatga aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag
      421 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
      481 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
      541 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
      601 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa tttcgtatgg
      661 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgtttt
//
