LOCUS       sequence-140634-         612 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression of mRIPK3 mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 612)
  TITLE     Francis Chan lab plasmids
REFERENCE   2  (bases 1 to 612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HA-mRIPK3 K51A-Flag/cHA-pTRIPZ vector
FEATURES             Location/Qualifiers
     source          1..612
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(97..123)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(133..156)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(254..265)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 gctcgctgcg cccttcgtct gacgtggcag cgctcgccgt gaggaggggg gcgcccgcgg
       61 gaggcgccaa aacccggcgc ggaggccacg cgtttaagcg taatctggaa catcgtatgg
      121 gtactcgagc tacttgtcat cgtcgtcctt gtagtccttg tggaagggct gccagcccct
      181 acccctgccg aactgtgctt ggtcatactt ggccgaactt gaggcagtag ttcttggtgg
      241 tgctaccaag gagttgtagt tcccaatctg cacttcagaa cagttgttga agacgagagc
      301 cggtggccct gtcattggat tcggtggggt ccagggatac caaggagtgc cgtgtcttcc
      361 atctccctga ttcctttggg ggtgaggacc gggagtctca gtaaagactg gcccaggtgt
      421 tgtgcctctg aagggtaaag tatgtggaat ctgaggagtg ccagccacgg ggtcagaaga
      481 tgtccttgct ggtgtggcag gcccaactga tgtgtcctgt gcttgcctct caggacattt
      541 tccaggaact ggtccggagg gttcctccaa atgcaggcgg tccagcattt tagaaaccat
      601 ggtttccctc gg
//
