LOCUS       sequence-140635-         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression of mRIPK3 mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  TITLE     Francis Chan lab plasmids
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HA-mRIPK3 T231A/S232A-Flag/cHA-pTRIPZ vector
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(100..126)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(136..159)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(257..268)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 gacgctcgct gcgcccttcg tctgacgtgg cagcgctcgc cgtgaggagg ggggcgcccg
       61 cgggaggcgc caaaacccgg cgcggaggcc acgcgtttaa gcgtaatctg gaacatcgta
      121 tgggtactcg agctacttgt catcgtcgtc cttgtagtcc ttgtggaagg gctgccagcc
      181 cctacccctg ccgaactgtg cttggtcata cttggccgaa cttgaggcag tagttcttgg
      241 tggtgctacc aaggagttgt agttcccaat ctgcacttca gaacagttgt tgaagacgag
      301 agccggtggc cctgtcattg gattcggtgg ggtccaggga taccaaggag tgccgtgtct
      361 tccatctccc tgattccttt gggggtgagg accgggagtc tcagtaaaga ctggcccagg
      421 tgttgtgcct ctgaagggta aagtatgtgg aatctgagga gtgccagcca cggggtcaga
      481 agatgtcctt gctggtgtgg caggcccaac tgatgtgtcc tgtgcttgcc tctcaggaca
      541 ttttccagga actggtccgg agggttcctc caaatgcagg cggtccagca ttttagaaac
      601 catggtttcc ctcgggcaat ccatttctgt gcctctttgg cttggctctc tggcagacaa
      661 gtttctgcca ctgcttctgt gctgagacag ataatgcttt acctcggaga cagcagcatc
      721 taccttgtcc tttaccagat tgtaaacttc attggttttt ggttcgcagt cctggaagga
      781 tggcctgttt
//
